000124460 001__ 124460
000124460 005__ 20241125101151.0
000124460 0247_ $$2doi$$a10.1021/acsapm.2c01980
000124460 0248_ $$2sideral$$a132903
000124460 037__ $$aART-2023-132903
000124460 041__ $$aeng
000124460 100__ $$0(orcid)0000-0002-1647-8207$$aPamplona, Regina
000124460 245__ $$aTuning of Mechanical Properties in Photopolymerizable Gelatin-Based Hydrogels for <i>In Vitro</i> Cell Culture Systems
000124460 260__ $$c2023
000124460 5060_ $$aAccess copy available to the general public$$fUnrestricted
000124460 5203_ $$aThe mechanical microenvironment plays a crucial role in the evolution of colorectal cancer, a complex disease characterized by heterogeneous tumors with varying elasticity. Toward setting up distinct scenarios, herein, we describe the preparation and characterization of gelatin methacrylamide (GelMA)-based hydrogels via two different mechanisms: free-radical photopolymerization and photo-induced thiol-ene reaction. A precise stiffness modulation of covalently crosslinked scaffolds was achieved through the application of well-defined irradiation times while keeping the intensity constant. Besides, the incorporation of thiol chemistry strongly increased stiffness with low to moderate curing times. This wide range of finely tuned mechanical properties successfully covered from healthy tissue to colorectal cancer stages. Hydrogels prepared in phosphate-buffered saline or Dulbecco’s modified Eagle’s medium resulted in different mechanical and swelling properties, although a similar trend was observed for both conditions: thiol-ene systems exhibited higher stiffness and, at the same time, higher swelling capacity than free-radical photopolymerized networks. In terms of biological behavior, three of the substrates showed good cell proliferation rates according to the formation of a confluent monolayer of Caco-2 cells after 14 days of cell culture. Likewise, a characteristic apical-basal polarization of cells was observed for these three hydrogels. These results demonstrate the versatility of the presented platform of biomimetic materials as in vitro cell culture scaffolds.
000124460 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/E15-20R$$9info:eu-repo/grantAgreement/ES/DGA-FSE/E47-20R$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/PGC2018-097583-B-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-109333RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00
000124460 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000124460 590__ $$a4.5$$b2023
000124460 592__ $$a0.982$$b2023
000124460 591__ $$aPOLYMER SCIENCE$$b22 / 95 = 0.232$$c2023$$dQ1$$eT1
000124460 593__ $$aOrganic Chemistry$$c2023$$dQ1
000124460 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b143 / 439 = 0.326$$c2023$$dQ2$$eT1
000124460 593__ $$aProcess Chemistry and Technology$$c2023$$dQ1
000124460 593__ $$aPolymers and Plastics$$c2023$$dQ1
000124460 594__ $$a7.2$$b2023
000124460 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000124460 700__ $$aGonzález-Lana, Sandra
000124460 700__ $$0(orcid)0000-0003-1378-0571$$aRomero, Pilar
000124460 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, Ignacio$$uUniversidad de Zaragoza
000124460 700__ $$0(orcid)0000-0003-0702-8260$$aMartín-Rapún, Rafael$$uUniversidad de Zaragoza
000124460 700__ $$0(orcid)0000-0003-3900-2866$$aSánchez-Somolinos, Carlos
000124460 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000124460 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000124460 773__ $$g5, 2 (2023), 1487-1498$$pACS appl. polymer. mater.$$tACS applied polymer materials$$x2637-6105
000124460 8564_ $$s6396490$$uhttps://zaguan.unizar.es/record/124460/files/texto_completo.pdf$$yVersión publicada
000124460 8564_ $$s3191821$$uhttps://zaguan.unizar.es/record/124460/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000124460 909CO $$ooai:zaguan.unizar.es:124460$$particulos$$pdriver
000124460 951__ $$a2024-11-22-12:06:54
000124460 980__ $$aARTICLE