124460 20241125101151.0 doi 10.1021/acsapm.2c01980 sideral 132903 ART-2023-132903 eng Pamplona, Regina (orcid)0000-0002-1647-8207 Tuning of Mechanical Properties in Photopolymerizable Gelatin-Based Hydrogels for <i>In Vitro</i> Cell Culture Systems 2023 Access copy available to the general public Unrestricted The mechanical microenvironment plays a crucial role in the evolution of colorectal cancer, a complex disease characterized by heterogeneous tumors with varying elasticity. Toward setting up distinct scenarios, herein, we describe the preparation and characterization of gelatin methacrylamide (GelMA)-based hydrogels via two different mechanisms: free-radical photopolymerization and photo-induced thiol-ene reaction. A precise stiffness modulation of covalently crosslinked scaffolds was achieved through the application of well-defined irradiation times while keeping the intensity constant. Besides, the incorporation of thiol chemistry strongly increased stiffness with low to moderate curing times. This wide range of finely tuned mechanical properties successfully covered from healthy tissue to colorectal cancer stages. Hydrogels prepared in phosphate-buffered saline or Dulbecco’s modified Eagle’s medium resulted in different mechanical and swelling properties, although a similar trend was observed for both conditions: thiol-ene systems exhibited higher stiffness and, at the same time, higher swelling capacity than free-radical photopolymerized networks. In terms of biological behavior, three of the substrates showed good cell proliferation rates according to the formation of a confluent monolayer of Caco-2 cells after 14 days of cell culture. Likewise, a characteristic apical-basal polarization of cells was observed for these three hydrogels. These results demonstrate the versatility of the presented platform of biomimetic materials as in vitro cell culture scaffolds. info:eu-repo/grantAgreement/ES/DGA-FSE/E15-20R info:eu-repo/grantAgreement/ES/DGA-FSE/E47-20R info:eu-repo/grantAgreement/ES/MICINN-FEDER/PGC2018-097583-B-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2019-109333RB-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.5 2023 POLYMER SCIENCE 22 / 95 = 0.232 2023 Q1 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 143 / 439 = 0.326 2023 Q2 T1 0.982 2023 Organic Chemistry 2023 Q1 Process Chemistry and Technology 2023 Q1 Polymers and Plastics 2023 Q1 7.2 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion González-Lana, Sandra Romero, Pilar (orcid)0000-0003-1378-0571 Ochoa, Ignacio Universidad de Zaragoza (orcid)0000-0003-2410-5678 Martín-Rapún, Rafael Universidad de Zaragoza (orcid)0000-0003-0702-8260 Sánchez-Somolinos, Carlos (orcid)0000-0003-3900-2866 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 5, 2 (2023), 1487-1498 ACS appl. polymer. mater. ACS applied polymer materials 2637-6105 6396490 http://zaguan.unizar.es/record/124460/files/texto_completo.pdf Versión publicada 3191821 http://zaguan.unizar.es/record/124460/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:124460 articulos driver 2024-11-22-12:06:54 ARTICLE