doi:10.1016/j.mtchem.2023.101404engBonet-Aleta, J.Hueso, J.L.Sánchez-Uriel, L.Encinas-Giménez, M.Irusta, S.Martín-Duque, P.Martínez, G.Santamaría, J.Synergistic assembly of gold and copper-iron oxide nanocatalysts to promote the simultaneous depletion of glucose and glutathioneART-2023-132963Glucose and glutathione (GSH) are key biomolecules for the regulation and growth of tumor cells. The use of inorganic nanocatalysts in biomedicine to target and deplete such specific molecules represents a novel and promising strategy against cancer. In this work, we present a ternary assembled nanohybrid based on Au and CuFe2O4 with the capability to simultaneously deplete glucose and GSH and generate reactive oxidative species (ROS) in a cascade process. We describe an example of a synergistic heterogeneous nanoarchitecture able to maintain the glucose oxidase-like activity of Au while preventing its deactivation in the presence of GSH. Au sites remain active due to the rapid response of the Cu–Fe co-catalyst to deplete GSH levels. This example of hybrid heterostructure represents an appealing alternative with dual-activity within the tumor microenvironment (TME) for potential anticancer therapy.2023http://zaguan.unizar.es/record/12527210.1016/j.mtchem.2023.101404http://zaguan.unizar.es/record/125272oai:zaguan.unizar.es:125272info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCEThis project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCEinfo:eu-repo/grantAgreement/ES/UZ/ICTS NANBIOSIS-CIBER-BBNMaterials Today Chemistry 29 (2023), 101404 [8 pp.]by-nc-ndhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccess