000125301 001__ 125301
000125301 005__ 20241125101151.0
000125301 0247_ $$2doi$$a10.1039/d2bm01835g
000125301 0248_ $$2sideral$$a132941
000125301 037__ $$aART-2023-132941
000125301 041__ $$aeng
000125301 100__ $$aBhandari, M.
000125301 245__ $$aSubcellular localization and therapeutic efficacy of polymeric micellar nanoparticles encapsulating bedaquiline for tuberculosis treatment in zebrafish
000125301 260__ $$c2023
000125301 5060_ $$aAccess copy available to the general public$$fUnrestricted
000125301 5203_ $$aThe combination drug regimens that have long been used to treat tuberculosis (TB), caused by Mycobacterium tuberculosis, are fraught with problems such as frequent administration, long duration of treatment, and harsh adverse effects, leading to the emergence of multidrug resistance. Moreover, there is no effective preventive vaccine against TB infection. In this context, nanoparticles (NPs) have emerged as a potential alternative method for drug delivery. Encapsulating antibiotics in biodegradable NPs has been shown to provide effective therapy and reduced toxicity against M. tuberculosis in different mammalian models, when compared to conventional free drug administration. Here, we evaluate the localization, therapeutic efficacy and toxic effects of polymeric micellar NPs encapsulating a promising but highly hydrophobic and toxic antitubercular drug bedaquiline (BQ) in zebrafish embryos infected with Mycobacterium marinum. Our study shows that the NP formulation of BQ improves survival and reduces bacterial burden in the infected embryos after treatment when compared to its free form. The intravenously injected BQ NPs have short circulation times due to their rapid and efficient uptake into the endothelial cells, as observed by correlative light and electron microscopy (CLEM).
000125301 536__ $$9info:eu-repo/grantAgreement/ES/MEC/FPU2016-02456$$9info:eu-repo/grantAgreement/ES/DGA/E15-20R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-109333RB-I00
000125301 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000125301 590__ $$a5.8$$b2023
000125301 592__ $$a1.206$$b2023
000125301 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b12 / 53 = 0.226$$c2023$$dQ1$$eT1
000125301 593__ $$aMaterials Science (miscellaneous)$$c2023$$dQ1
000125301 593__ $$aBiomedical Engineering$$c2023$$dQ1
000125301 594__ $$a11.5$$b2023
000125301 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000125301 700__ $$0(orcid)0000-0002-5823-0314$$aSoria-Carrera, H.
000125301 700__ $$aWohlmann, J.
000125301 700__ $$aKnudsen Dal, N. J.
000125301 700__ $$0(orcid)0000-0003-1081-8482$$aMartínez de la Fuente, J.
000125301 700__ $$0(orcid)0000-0003-0702-8260$$aMartín-Rapún, R.$$uUniversidad de Zaragoza
000125301 700__ $$aGriffiths, G.
000125301 700__ $$aFenaroli, F.
000125301 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000125301 773__ $$g11, 6 (2023), 2103-2114$$pBiomater. sci.$$tBiomaterials Science$$x2047-4830
000125301 8564_ $$s8238436$$uhttps://zaguan.unizar.es/record/125301/files/texto_completo.pdf$$yVersión publicada
000125301 8564_ $$s2740368$$uhttps://zaguan.unizar.es/record/125301/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000125301 909CO $$ooai:zaguan.unizar.es:125301$$particulos$$pdriver
000125301 951__ $$a2024-11-22-12:06:55
000125301 980__ $$aARTICLE