000125750 001__ 125750
000125750 005__ 20230420165728.0
000125750 0247_ $$2doi$$a10.1016/j.ijbiomac.2023.124089
000125750 0248_ $$2sideral$$a133138
000125750 037__ $$aART-2023-133138
000125750 041__ $$aeng
000125750 100__ $$0(orcid)0000-0001-7460-5916$$aLostao, Anabel
000125750 245__ $$aRecent advances in sensing the inter-biomolecular interactions at the nanoscale – A comprehensive review of AFM-based force spectroscopy
000125750 260__ $$c2023
000125750 5060_ $$aAccess copy available to the general public$$fUnrestricted
000125750 5203_ $$aBiomolecular interactions underpin most processes inside the cell. Hence, a precise and quantitative understanding of molecular association and dissociation events is crucial, not only from a fundamental perspective, but also for the rational design of biomolecular platforms for state-of-the-art biomedical and industrial applications. In this context, atomic force microscopy (AFM) appears as an invaluable experimental technique, allowing the measurement of the mechanical strength of biomolecular complexes to provide a quantitative characterization of their interaction properties from a single molecule perspective. In the present review, the most recent methodological advances in this field are presented with special focus on bioconjugation, immobilization and AFM tip functionalization, dynamic force spectroscopy measurements, molecular recognition imaging and theoretical modeling. We expect this work to significantly aid in grasping the principles of AFM-based force spectroscopy (AFM-FS) technique and provide the necessary tools to acquaint the type of data that can be achieved from this type of experiments. Furthermore, a critical assessment is done with other nanotechnology techniques to better visualize the future prospects of AFM-FS.
000125750 536__ $$9info:eu-repo/grantAgreement/ES/CSIC/QTP-2103003$$9info:eu-repo/grantAgreement/ES/DGA/E35-20R$$9info:eu-repo/grantAgreement/ES/DGA/LMP58-18$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PID2019-103901GB-I00
000125750 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000125750 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000125750 700__ $$aLim, KeeSiang
000125750 700__ $$aPallarés, María Carmen
000125750 700__ $$aPtak, Arkadiusz
000125750 700__ $$0(orcid)0000-0003-3459-8605$$aMarcuello, Carlos
000125750 773__ $$g238 (2023), 124089 [19 pp.]$$pInt. j. biol. macromol.$$tINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES$$x0141-8130
000125750 8564_ $$s4397477$$uhttps://zaguan.unizar.es/record/125750/files/texto_completo.pdf$$yVersión publicada
000125750 8564_ $$s2714364$$uhttps://zaguan.unizar.es/record/125750/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000125750 909CO $$ooai:zaguan.unizar.es:125750$$particulos$$pdriver
000125750 951__ $$a2023-04-20-14:35:24
000125750 980__ $$aARTICLE