000125781 001__ 125781 000125781 005__ 20241125101135.0 000125781 0247_ $$2doi$$a10.1007/s10096-023-04579-9 000125781 0248_ $$2sideral$$a133171 000125781 037__ $$aART-2023-133171 000125781 041__ $$aeng 000125781 100__ $$aAbdullahi, Idris Nasir 000125781 245__ $$aNasotracheal enterococcal carriage and resistomes: detection of optrA-, poxtA- and cfrD-carrying strains in migratory birds, livestock, pets, and in-contact humans in Spain 000125781 260__ $$c2023 000125781 5060_ $$aAccess copy available to the general public$$fUnrestricted 000125781 5203_ $$aThis study determined the carriage rates and antimicrobial resistance (AMR) genes of enterococci from nasotracheal samples of three healthy animal species and in-contact humans. Nasal samples were collected from 27 dog-owning households (34 dogs, 41 humans) and 4 pig-farms (40 pigs, 10 pig-farmers), and they were processed for enterococci recovery (MALDI-TOF–MS identification). Also, a collection of 144 enterococci previously recovered of tracheal/nasal samples from 87 white stork nestlings were characterized. The AMR phenotypes were determined in all enterococci and AMR genes were studied by PCR/sequencing. MultiLocus-Sequence-Typing was performed for selected isolates. About 72.5% and 60% of the pigs and pig-farmers, and 29.4% and 4.9%, of healthy dogs and owners were enterococci nasal carriers, respectively. In storks, 43.5% of tracheal and 69.2% of nasal samples had enterococci carriages. Enterococci carrying multidrug-resistance phenotype was identified in 72.5%/40.0%/50.0%/23.5%/1.1% of pigs/pig-farmers/dogs/dogs’ owners/storks, respectively. Of special relevance was the detection of linezolid-resistant enterococci (LRE) in (a) 33.3% of pigs (E. faecalis-carrying optrA and/or cfrD of ST59, ST330 or ST474 lineages; E. casseliflavus-carrying optrA and cfrD); (b) 10% of pig farmers (E. faecalis-ST330-carrying optrA); (c) 2.9% of dogs (E. faecalis-ST585-carrying optrA); and (d) 1.7% of storks (E. faecium-ST1736-carrying poxtA). The fexA gene was found in all optrA-positive E. faecalis and E. casseliflavus isolates, while fexB was detected in the poxtA-positive E. faecium isolate. The enterococci diversity and AMR rates from the four hosts reflect differences in antimicrobial selection pressure. The detection of LRE carrying acquired and transferable genes in all the hosts emphasizes the need to monitor LRE using a One-Health approach. 000125781 536__ $$9info:eu-repo/grantAgreement/ES/MCIN/AEI/PID2019-106158RB-I00$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalent$$9info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent 000125781 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000125781 590__ $$a3.7$$b2023 000125781 592__ $$a1.02$$b2023 000125781 591__ $$aMICROBIOLOGY$$b61 / 161 = 0.379$$c2023$$dQ2$$eT2 000125781 593__ $$aInfectious Diseases$$c2023$$dQ1 000125781 591__ $$aINFECTIOUS DISEASES$$b39 / 132 = 0.295$$c2023$$dQ2$$eT1 000125781 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1 000125781 593__ $$aMicrobiology (medical)$$c2023$$dQ2 000125781 594__ $$a10.4$$b2023 000125781 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000125781 700__ $$aLozano, Carmen 000125781 700__ $$aJuárez-Fernández, Guillermo 000125781 700__ $$aHöfle, Ursula 000125781 700__ $$0(orcid)0000-0002-4180-1539$$aSimón, Carmen$$uUniversidad de Zaragoza 000125781 700__ $$aRueda, Silvia 000125781 700__ $$aMartínez, Angela 000125781 700__ $$aÁlvarez-Martínez, Sandra 000125781 700__ $$aEguizábal, Paula 000125781 700__ $$aMartínez-Cámara, Beatriz 000125781 700__ $$aZarazaga, Myriam 000125781 700__ $$aTorres, Carmen 000125781 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000125781 773__ $$g42, 5 (2023), 569-581$$pEur. j. clin. microbiol. infect. dis.$$tEUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES$$x0934-9723 000125781 8564_ $$s907349$$uhttps://zaguan.unizar.es/record/125781/files/texto_completo.pdf$$yVersión publicada 000125781 8564_ $$s2303040$$uhttps://zaguan.unizar.es/record/125781/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000125781 909CO $$ooai:zaguan.unizar.es:125781$$particulos$$pdriver 000125781 951__ $$a2024-11-22-12:00:33 000125781 980__ $$aARTICLE