125781 20241125101135.0 doi 10.1007/s10096-023-04579-9 sideral 133171 ART-2023-133171 eng Abdullahi, Idris Nasir Nasotracheal enterococcal carriage and resistomes: detection of optrA-, poxtA- and cfrD-carrying strains in migratory birds, livestock, pets, and in-contact humans in Spain 2023 Access copy available to the general public Unrestricted This study determined the carriage rates and antimicrobial resistance (AMR) genes of enterococci from nasotracheal samples of three healthy animal species and in-contact humans. Nasal samples were collected from 27 dog-owning households (34 dogs, 41 humans) and 4 pig-farms (40 pigs, 10 pig-farmers), and they were processed for enterococci recovery (MALDI-TOF–MS identification). Also, a collection of 144 enterococci previously recovered of tracheal/nasal samples from 87 white stork nestlings were characterized. The AMR phenotypes were determined in all enterococci and AMR genes were studied by PCR/sequencing. MultiLocus-Sequence-Typing was performed for selected isolates. About 72.5% and 60% of the pigs and pig-farmers, and 29.4% and 4.9%, of healthy dogs and owners were enterococci nasal carriers, respectively. In storks, 43.5% of tracheal and 69.2% of nasal samples had enterococci carriages. Enterococci carrying multidrug-resistance phenotype was identified in 72.5%/40.0%/50.0%/23.5%/1.1% of pigs/pig-farmers/dogs/dogs’ owners/storks, respectively. Of special relevance was the detection of linezolid-resistant enterococci (LRE) in (a) 33.3% of pigs (E. faecalis-carrying optrA and/or cfrD of ST59, ST330 or ST474 lineages; E. casseliflavus-carrying optrA and cfrD); (b) 10% of pig farmers (E. faecalis-ST330-carrying optrA); (c) 2.9% of dogs (E. faecalis-ST585-carrying optrA); and (d) 1.7% of storks (E. faecium-ST1736-carrying poxtA). The fexA gene was found in all optrA-positive E. faecalis and E. casseliflavus isolates, while fexB was detected in the poxtA-positive E. faecium isolate. The enterococci diversity and AMR rates from the four hosts reflect differences in antimicrobial selection pressure. The detection of LRE carrying acquired and transferable genes in all the hosts emphasizes the need to monitor LRE using a One-Health approach. info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalent info:eu-repo/grantAgreement/ES/MCIN/AEI/PID2019-106158RB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.7 2023 MICROBIOLOGY 61 / 161 = 0.379 2023 Q2 T2 INFECTIOUS DISEASES 39 / 132 = 0.295 2023 Q2 T1 1.02 2023 Infectious Diseases 2023 Q1 Medicine (miscellaneous) 2023 Q1 Microbiology (medical) 2023 Q2 10.4 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Lozano, Carmen Juárez-Fernández, Guillermo Höfle, Ursula Simón, Carmen Universidad de Zaragoza (orcid)0000-0002-4180-1539 Rueda, Silvia Martínez, Angela Álvarez-Martínez, Sandra Eguizábal, Paula Martínez-Cámara, Beatriz Zarazaga, Myriam Torres, Carmen 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 42, 5 (2023), 569-581 Eur. j. clin. microbiol. infect. dis. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES 0934-9723 907349 http://zaguan.unizar.es/record/125781/files/texto_completo.pdf Versión publicada 2303040 http://zaguan.unizar.es/record/125781/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:125781 articulos driver 2024-11-22-12:00:33 ARTICLE