000125813 001__ 125813
000125813 005__ 20241125101138.0
000125813 0247_ $$2doi$$a10.1177/1098612X221150625
000125813 0248_ $$2sideral$$a133173
000125813 037__ $$aART-2023-133173
000125813 041__ $$aeng
000125813 100__ $$aAlgorta, A.
000125813 245__ $$aIsolation and characterization of feline dental pulp stem cells
000125813 260__ $$c2023
000125813 5060_ $$aAccess copy available to the general public$$fUnrestricted
000125813 5203_ $$aObjectives The aim of this study was to isolate feline dental pulp stem cells (fDPSCs) and characterize their clonogenic and proliferative abilities, as well as their multipotency, immunophenotype and cytogenetic stability. Methods Dental pulp was isolated by explant culture from two cats <1 year old at post mortem. Their clonogenicity was characterized using a colony-forming unit fibroblast assay, and their proliferative ability was quantified with a doubling time assay in passages 2, 4 and 6 (P2, P4 and P6, respectively). Multipotency was characterized with an in vitro trilineage differentiation assay in P2, and cells were immunophenotyped in P4 by flow cytometry. Chromosomic stability was evaluated by cytogenetic analysis in P2, P4 and P6. Results The fDPSCs displayed spindle and epithelial-like morphologies. Isolated cells showed a marked clonogenic capacity and doubling time was maintained from P2 to P6. Trilineage differentiation was obtained in one sample, while the other showed osteogenic and chondrogenic differentiation. Immunophenotypic analysis showed fDPSCs were CD45−, CD90+ and CD44+. Structural and numerical cytogenetic aberrations were observed in P2–P4. Conclusions and relevance In this study, fDPSCs from two cats were isolated by explant culture and immunophenotyped. Cells displayed clonogenic and proliferative ability, and multipotency in vitro, and signs of chromosomic instability were observed. Although a larger study is needed to confirm these results, this is the first report of fDPSC isolation and in vitro characterization.
000125813 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000125813 590__ $$a1.9$$b2023
000125813 592__ $$a0.831$$b2023
000125813 591__ $$aVETERINARY SCIENCES$$b46 / 167 = 0.275$$c2023$$dQ2$$eT1
000125813 593__ $$aSmall Animals$$c2023$$dQ1
000125813 594__ $$a3.9$$b2023
000125813 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/publishedVersion
000125813 700__ $$aArtigas, R.
000125813 700__ $$aRial, A.
000125813 700__ $$aBrandl, S.
000125813 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, C.$$uUniversidad de Zaragoza
000125813 700__ $$aBenavides, U.
000125813 700__ $$aMaisonnave, J.
000125813 700__ $$aYaneselli, K.
000125813 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000125813 773__ $$g25, 2 (2023), [9 pp.]$$pJ. Feline Med. Surg.$$tJOURNAL OF FELINE MEDICINE AND SURGERY$$x1098-612X
000125813 8564_ $$s1738549$$uhttps://zaguan.unizar.es/record/125813/files/texto_completo.pdf$$yVersión publicada
000125813 8564_ $$s2513533$$uhttps://zaguan.unizar.es/record/125813/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000125813 909CO $$ooai:zaguan.unizar.es:125813$$particulos$$pdriver
000125813 951__ $$a2024-11-22-12:01:48
000125813 980__ $$aARTICLE