125819 20241125101148.0 doi 10.3390/ani13050905 sideral 133379 ART-2023-133379 eng Peña-Delgado, Victoria Universidad de Zaragoza (orcid)0000-0002-4670-3735 Improvement of the seminal characteristics in rams using agri-food by-products rich in phytomelatonin 2023 Access copy available to the general public Unrestricted The aim of this study was to evaluate the effect of a phytomelatonin-rich diet, including by-products from the food industry, on ram sperm quality and seminal plasma composition. Melatonin content in several by-products before and after in vitro ruminal and abomasal digestion was determined by HPLC-ESI-MS/MS. Finally, 20% of a mix of grape pulp with pomegranate and tomato pomaces was included in the rams’ diet, constituting the phytomelatonin-rich diet. Feeding the rams with this diet resulted in an increase in seminal plasma melatonin levels compared with the control group (commercial diet) in the third month of the study. In addition, percentages higher than those in the control group of morphologically normal viable spermatozoa with a low content of reactive oxygen species were observed from the second month onwards. However, the antioxidant effect does not seem to be exerted through the modulation of the antioxidant enzymes since the analysis of the activities of catalase, glutathione reductase and glutathione peroxidase in seminal plasma revealed no significant differences between the two experimental groups. In conclusion, this study reveals, for the first time, that a phytomelatonin-rich diet can improve seminal characteristics in rams. info:eu-repo/grantAgreement/ES/DGA/A07-20R info:eu-repo/grantAgreement/ES/MINECO/AGL2017-83799-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.7 2023 VETERINARY SCIENCES 16 / 167 = 0.096 2023 Q1 T1 AGRICULTURE, DAIRY & ANIMAL SCIENCE 10 / 80 = 0.125 2023 Q1 T1 0.698 2023 Veterinary (miscellaneous) 2023 Q1 Animal Science and Zoology 2023 Q1 4.9 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Carvajal-Serna, Melissa Universidad de Zaragoza (orcid)0000-0003-3929-5064 Fondevila, Manuel Universidad de Zaragoza (orcid)0000-0002-0712-1185 Martín-Cabrejas, María A. Aguilera, Yolanda Álvarez-Rivera, Gerardo Abecia, José A. Universidad de Zaragoza (orcid)0000-0003-2827-3054 Casao, Adriana Universidad de Zaragoza (orcid)0000-0003-1997-4262 Pérez-Pe, Rosaura Universidad de Zaragoza (orcid)0000-0002-2312-6402 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 13, 5 (2023), 905 [15 pp.] Animals (Basel) Animals 2076-2615 1928186 http://zaguan.unizar.es/record/125819/files/texto_completo.pdf Versión publicada 2520562 http://zaguan.unizar.es/record/125819/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:125819 articulos driver 2024-11-22-12:05:26 ARTICLE