000125829 001__ 125829
000125829 005__ 20241125101150.0
000125829 0247_ $$2doi$$a10.1016/j.isci.2023.106411
000125829 0248_ $$2sideral$$a133196
000125829 037__ $$aART-2023-133196
000125829 041__ $$aeng
000125829 100__ $$avan Wijk, Rob C.
000125829 245__ $$aImplementing best practises on data generation and reporting of Mycobacterium tuberculosis in vitro assays within the ERA4TB consortium
000125829 260__ $$c2023
000125829 5060_ $$aAccess copy available to the general public$$fUnrestricted
000125829 5203_ $$aTuberculosis (TB) is the historical leading cause of death by a single infectious agent. The European Regimen Accelerator for Tuberculosis (ERA4TB) is a public-private partnership of 30+ institutions with the objective to progress new anti-TB regimens into the clinic. Thus, robust and replicable results across independent laboratories are essential for reliable interpretation of treatment efficacy. A standardization workgroup unified in vitro protocols and data reporting templates. Time-kill assays provide essential input data for pharmacometric model-informed translation of single agents and regimens activity from in vitro to in vivo and the clinic. Five conditions were assessed by time-kill assays in six independent laboratories using four bacterial plating methods. Baseline bacterial burden varied between laboratories but variability was limited in net drug effect, confirming 2.5 μL equally robust as 100 μL plating. This exercise establishes the foundations of collaborative data generation, reporting, and integration within the overarching Antimicrobial Resistance Accelerator program.
000125829 536__ $$9info:eu-repo/grantAgreement/EC/H2020/853989/EU/EUROPEAN REGIMEN ACCELERATOR FOR TUBERCULOSIS/ERA4TB$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 853989-ERA4TB
000125829 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000125829 590__ $$a4.6$$b2023
000125829 592__ $$a1.497$$b2023
000125829 591__ $$aMULTIDISCIPLINARY SCIENCES$$b19 / 134 = 0.142$$c2023$$dQ1$$eT1
000125829 593__ $$aMultidisciplinary$$c2023$$dQ1
000125829 594__ $$a7.2$$b2023
000125829 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000125829 700__ $$0(orcid)0000-0002-0111-4697$$aLucía, Ainhoa$$uUniversidad de Zaragoza
000125829 700__ $$aSudhakar, Pavan Kumar
000125829 700__ $$aSonnenkalb, Lindsay
000125829 700__ $$aGaudin, Cyril
000125829 700__ $$aHoffmann, Eik
000125829 700__ $$aDremierre, Bérénice
000125829 700__ $$aAguilar-Ayala, Diana Angélica$$uUniversidad de Zaragoza
000125829 700__ $$aDal Molin, Michael
000125829 700__ $$aRybniker, Jan
000125829 700__ $$ade Giorgi, Stefano
000125829 700__ $$aCioetto-Mazzabò, Laura
000125829 700__ $$aSegafreddo, Greta
000125829 700__ $$aManganelli, Riccardo
000125829 700__ $$aDegiacomi, Giulia
000125829 700__ $$aRecchia, Deborah
000125829 700__ $$aPasca, Maria Rosalia
000125829 700__ $$aSimonsson, Ulrika S. H.
000125829 700__ $$0(orcid)0000-0002-8480-0325$$aRamón-García, Santiago
000125829 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000125829 773__ $$g26, 4 (2023), 106411 [15 pp.]$$piScience$$tISCIENCE$$x2589-0042
000125829 8564_ $$s3999249$$uhttps://zaguan.unizar.es/record/125829/files/texto_completo.pdf$$yVersión publicada
000125829 8564_ $$s1029444$$uhttps://zaguan.unizar.es/record/125829/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000125829 909CO $$ooai:zaguan.unizar.es:125829$$particulos$$pdriver
000125829 951__ $$a2024-11-22-12:06:24
000125829 980__ $$aARTICLE