125861 20241125101203.0 doi 10.1021/acsami.2c20566 sideral 133188 ART-2023-133188 eng Sancho-Albero, María Universidad de Zaragoza (orcid)0000-0001-8762-5457 Superfluorinated extracellular vesicles for in vivo imaging by 19f-mri 2023 Access copy available to the general public Unrestricted Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication and have great potential as efficient delivery vectors. However, a better understanding of EV in vivo behavior is hampered by the limitations of current imaging tools. In addition, chemical labels present the risk of altering the EV membrane features and, thus, in vivo behavior. 19F-MRI is a safe bioimaging technique providing selective images of exogenous probes. Here, we present the first example of fluorinated EVs containing PERFECTA, a branched molecule with 36 magnetically equivalent 19F atoms. A PERFECTA emulsion is given to the cells, and PERFECTA-containing EVs are naturally produced. PERFECTA-EVs maintain the physicochemical features, morphology, and biological fingerprint as native EVs but exhibit an intense 19F-NMR signal and excellent 19F relaxation times. In vivo 19F-MRI and tumor-targeting capabilities of stem cell-derived PERFECTA-EVs are also proved. We propose PERFECTA-EVs as promising biohybrids for imaging biodistribution and delivery of EVs throughout the body. info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN info:eu-repo/grantAgreement/ES/MEC/FPU15-03419 info:eu-repo/grantAgreement/ES/MINECO/Consolider-Ingenio2010 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 8.5 2023 NANOSCIENCE & NANOTECHNOLOGY 28 / 141 = 0.199 2023 Q1 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 68 / 439 = 0.155 2023 Q1 T1 2.058 2023 Materials Science (miscellaneous) 2023 Q1 Nanoscience and Nanotechnology 2023 Q1 Medicine (miscellaneous) 2023 Q1 16.0 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Ayaz, Nazeeha Sebastian, Victor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Chirizzi, Cristina Encinas-Gimenez, Miguel Universidad de Zaragoza Neri, Giulia Chaabane, Linda Luján, Lluís Universidad de Zaragoza (orcid)0000-0002-2053-9842 Martin-Duque, Pilar Metrangolo, Pierangelo Santamaría, Jesús Universidad de Zaragoza (orcid)0000-0002-8701-9745 Baldelli Bombelli, Francesca 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 15 (2023), 8974-8985 ACS appl. mater. interfaces ACS applied materials & interfaces 1944-8244 9944759 http://zaguan.unizar.es/record/125861/files/texto_completo.pdf Versión publicada 2782234 http://zaguan.unizar.es/record/125861/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:125861 articulos driver 2024-11-22-12:12:39 ARTICLE