000125919 001__ 125919
000125919 005__ 20241125101138.0
000125919 0247_ $$2doi$$a10.3390/ijms24043932
000125919 0248_ $$2sideral$$a133440
000125919 037__ $$aART-2023-133440
000125919 041__ $$aeng
000125919 100__ $$aHedhly, A.
000125919 245__ $$aS-Locus genotyping in Japanese plum by high throughput sequencing using a synthetic S-loci reference sequence
000125919 260__ $$c2023
000125919 5060_ $$aAccess copy available to the general public$$fUnrestricted
000125919 5203_ $$aSelf-incompatibility in Prunus species is governed by a single locus consisting of two highly multi-allelic and tightly linked genes, one coding for an F-box protein—i.e., SFB in Prunus- controlling the pollen specificity and one coding for an S-RNase gene controlling the pistil specificity. Genotyping the allelic combination in a fruit tree species is an essential procedure both for cross-based breeding and for establishing pollination requirements. Gel-based PCR techniques using primer pairs designed from conserved regions and spanning polymorphic intronic regions are traditionally used for this task. However, with the great advance of massive sequencing techniques and the lowering of sequencing costs, new genotyping-by-sequencing procedures are emerging. The alignment of resequenced individuals to reference genomes, commonly used for polymorphism detection, yields little or no coverage in the S-locus region due to high polymorphism between different alleles within the same species, and cannot be used for this purpose. Using the available sequences of Japanese plum S-loci concatenated in a rosary-like structure as synthetic reference sequence, we describe a procedure to accurately genotype resequenced individuals that allowed the analysis of the S-genotype in 88 Japanese plum cultivars, 74 of them are reported for the first time. In addition to unraveling two new S-alleles from published reference genomes, we identified at least two S-alleles in 74 cultivars. According to their S-allele composition, they were assigned to 22 incompatibility groups, including nine new incompatibility groups reported here for the first time (XXVII-XXXV).
000125919 536__ $$9info:eu-repo/grantAgreement/ES/MCINN-AEI/PID2020-115473RR-I00$$9info:eu-repo/grantAgreement/ES/DGA/A12-17R
000125919 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000125919 590__ $$a4.9$$b2023
000125919 592__ $$a1.179$$b2023
000125919 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 313 = 0.211$$c2023$$dQ1$$eT1
000125919 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1
000125919 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b68 / 231 = 0.294$$c2023$$dQ2$$eT1
000125919 593__ $$aPhysical and Theoretical Chemistry$$c2023$$dQ1
000125919 593__ $$aComputer Science Applications$$c2023$$dQ1
000125919 593__ $$aInorganic Chemistry$$c2023$$dQ1
000125919 593__ $$aSpectroscopy$$c2023$$dQ1
000125919 593__ $$aOrganic Chemistry$$c2023$$dQ1
000125919 593__ $$aMolecular Biology$$c2023$$dQ2
000125919 593__ $$aCatalysis$$c2023$$dQ2
000125919 594__ $$a8.1$$b2023
000125919 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000125919 700__ $$aGuerra, M. E.
000125919 700__ $$aGrimplet, J.$$uUniversidad de Zaragoza
000125919 700__ $$0(orcid)0000-0002-8321-1764$$aRodrigo, J.
000125919 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000125919 773__ $$g24, 4 (2023), 3932 [18 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000125919 8564_ $$s1660932$$uhttps://zaguan.unizar.es/record/125919/files/texto_completo.pdf$$yVersión publicada
000125919 8564_ $$s2855902$$uhttps://zaguan.unizar.es/record/125919/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000125919 909CO $$ooai:zaguan.unizar.es:125919$$particulos$$pdriver
000125919 951__ $$a2024-11-22-12:01:34
000125919 980__ $$aARTICLE