000125952 001__ 125952
000125952 005__ 20241125101134.0
000125952 0247_ $$2doi$$a10.1007/s13105-023-00953-5
000125952 0248_ $$2sideral$$a133524
000125952 037__ $$aART-2023-133524
000125952 041__ $$aeng
000125952 100__ $$aNúñez, Sonia
000125952 245__ $$aAntidiabetic and anti-obesity properties of a polyphenol-rich flower extract from Tagetes Erecta L. and its effects on Caenorhabditis Elegans fat storages
000125952 260__ $$c2023
000125952 5060_ $$aAccess copy available to the general public$$fUnrestricted
000125952 5203_ $$aDiabetes mellitus (DM) is a metabolic disease characterized by a high blood sugar level that can cause severe complications to the organism or even death when not treated. However, certain dietary habits and foods may have beneficial effects on this condition. A polyphenolic-rich extract (containing hyperoside, isoquercitrin, quercetin, ellagic acid, and vanillic acid) of Tageres erecta L. (T. erecta) was obtained from yellow and orange flowers using an ethanolic Soxhlet extraction. These extracts were screened for antidiabetic and anti-obesity properties using in vitro and in vivo procedures. The capacity to inhibit the enzymes lipase and α-glucosidase, as well as the inhibition of advance glycation end-products (AGEs) was tested in vitro. Caenorhabditis elegans (C. elegans) was used as an obesity in vivo model to assess extracts effects on fat accumulation using the wild-type strain N2 and a mutant with no N3 fatty acid desaturase activity BX24. Extracts from both cultivars (yellow and orange) T. erecta presented in vitro inhibitory activity against the enzymes lipase and α-glucosidase, showing lower IC50 values than acarbose (control). They also showed important activity in preventing AGEs formation. The polyphenol-rich matrices reduced the fat content of obese worms in the wild-type strain (N2) down to levels of untreated C. elegans, with no significant differences found between negative control (100% reduction) and both tested samples (p < 0.05). Meanwhile, the fat reduction was considerably lower in the BX24 mutants (fat-1(wa-9)), suggesting that N3 fatty acid desaturase activity could be partially involved in the T. erecta flower effect. Our findings suggested that polyphenols from T. erecta can be considered candidate bioactive compounds in the prevention and improvement of metabolic chronic diseases such as obesity and diabetes.
000125952 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B44-20D$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2021-BIO-08
000125952 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000125952 590__ $$a3.7$$b2023
000125952 592__ $$a0.953$$b2023
000125952 591__ $$aPHYSIOLOGY$$b17 / 85 = 0.2$$c2023$$dQ1$$eT1
000125952 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1
000125952 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b119 / 313 = 0.38$$c2023$$dQ2$$eT2
000125952 593__ $$aPhysiology$$c2023$$dQ2
000125952 593__ $$aBiochemistry$$c2023$$dQ2
000125952 594__ $$a6.6$$b2023
000125952 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000125952 700__ $$aMoliner, Cristina
000125952 700__ $$0(orcid)0000-0002-2231-7565$$aValero, Marta Sofía$$uUniversidad de Zaragoza
000125952 700__ $$aMustafa, Ahmed M.
000125952 700__ $$aMaggi, Filippo
000125952 700__ $$aGómez-Rincón, Carlota
000125952 700__ $$0(orcid)0000-0001-6969-1055$$aLópez, Víctor
000125952 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000125952 773__ $$g79, 2 (2023), 427-440$$pJ. physiol. biochem.$$tJournal of Physiology and Biochemistry$$x1138-7548
000125952 8564_ $$s1577901$$uhttps://zaguan.unizar.es/record/125952/files/texto_completo.pdf$$yVersión publicada
000125952 8564_ $$s2158734$$uhttps://zaguan.unizar.es/record/125952/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000125952 909CO $$ooai:zaguan.unizar.es:125952$$particulos$$pdriver
000125952 951__ $$a2024-11-22-11:59:53
000125952 980__ $$aARTICLE