000125962 001__ 125962
000125962 005__ 20240705134147.0
000125962 0247_ $$2doi$$a10.1126/sciadv.ade9948
000125962 0248_ $$2sideral$$a133488
000125962 037__ $$aART-2023-133488
000125962 041__ $$aeng
000125962 100__ $$aLozano-Juste, Jorge
000125962 245__ $$aStructure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought
000125962 260__ $$c2023
000125962 5060_ $$aAccess copy available to the general public$$fUnrestricted
000125962 5203_ $$aStrategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of chemical ligands, which in turn can be optimized by structural information. Through structure-based targeted design, we have combined chemical and genetic approaches to generate an ABA receptor agonist molecule (iSB09) and engineer a CsPYL1 ABA receptor, named CsPYL15m, which efficiently binds iSB09. This optimized receptor-agonist pair leads to activation of ABA signaling and marked drought tolerance. No constitutive activation of ABA signaling and hence growth penalty was observed in transformed Arabidopsis thaliana plants. Therefore, conditional and efficient activation of ABA signaling was achieved through a chemical-genetic orthogonal approach based on iterative cycles of ligand and receptor optimization driven by the structure of ternary receptor-ligand-phosphatase complexes.
000125962 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000125962 592__ $$a4.483$$b2023
000125962 593__ $$aMultidisciplinary$$c2023$$dQ1
000125962 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000125962 700__ $$aInfantes, Lourdes
000125962 700__ $$aGarcia-Maquilon, Irene
000125962 700__ $$aRuiz-Partida, Rafael
000125962 700__ $$aMerilo, Ebe
000125962 700__ $$aBenavente, Juan Luis
000125962 700__ $$0(orcid)0000-0001-5702-4538$$aVelazquez-Campoy, Adrian$$uUniversidad de Zaragoza
000125962 700__ $$aCoego, Alberto
000125962 700__ $$aBono, Mar
000125962 700__ $$aForment, Javier
000125962 700__ $$aPampín, Begoña
000125962 700__ $$aDestito, Paolo
000125962 700__ $$aMonteiro, Adrián
000125962 700__ $$aRodríguez, Ramón
000125962 700__ $$aCruces, Jacobo
000125962 700__ $$aRodriguez, Pedro L.
000125962 700__ $$aAlbert, Armando
000125962 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000125962 773__ $$g9, 10 (2023), eade994 [18 pp.]$$pSci. adv.$$tScience advances$$x2375-2548
000125962 8564_ $$s2423645$$uhttps://zaguan.unizar.es/record/125962/files/texto_completo.pdf$$yVersión publicada
000125962 8564_ $$s3578693$$uhttps://zaguan.unizar.es/record/125962/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000125962 909CO $$ooai:zaguan.unizar.es:125962$$particulos$$pdriver
000125962 951__ $$a2024-07-05-12:47:42
000125962 980__ $$aARTICLE