125962 20241125101134.0 doi 10.1126/sciadv.ade9948 sideral 133488 ART-2023-133488 eng Lozano-Juste, Jorge Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought 2023 Access copy available to the general public Unrestricted Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of chemical ligands, which in turn can be optimized by structural information. Through structure-based targeted design, we have combined chemical and genetic approaches to generate an ABA receptor agonist molecule (iSB09) and engineer a CsPYL1 ABA receptor, named CsPYL15m, which efficiently binds iSB09. This optimized receptor-agonist pair leads to activation of ABA signaling and marked drought tolerance. No constitutive activation of ABA signaling and hence growth penalty was observed in transformed Arabidopsis thaliana plants. Therefore, conditional and efficient activation of ABA signaling was achieved through a chemical-genetic orthogonal approach based on iterative cycles of ligand and receptor optimization driven by the structure of ternary receptor-ligand-phosphatase complexes. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 11.7 2023 MULTIDISCIPLINARY SCIENCES 11 / 134 = 0.082 2023 Q1 T1 4.483 2023 Multidisciplinary 2023 Q1 21.4 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Infantes, Lourdes Garcia-Maquilon, Irene Ruiz-Partida, Rafael Merilo, Ebe Benavente, Juan Luis Velazquez-Campoy, Adrian Universidad de Zaragoza (orcid)0000-0001-5702-4538 Coego, Alberto Bono, Mar Forment, Javier Pampín, Begoña Destito, Paolo Monteiro, Adrián Rodríguez, Ramón Cruces, Jacobo Rodriguez, Pedro L. Albert, Armando 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 9, 10 (2023), eade994 [18 pp.] Sci. adv. Science advances 2375-2548 2423645 http://zaguan.unizar.es/record/125962/files/texto_completo.pdf Versión publicada 3578693 http://zaguan.unizar.es/record/125962/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:125962 articulos driver 2024-11-22-12:00:14 ARTICLE