000125967 001__ 125967
000125967 005__ 20241125101136.0
000125967 0247_ $$2doi$$a10.1038/s41598-023-32603-7
000125967 0248_ $$2sideral$$a133491
000125967 037__ $$aART-2023-133491
000125967 041__ $$aeng
000125967 100__ $$aVarela-Martínez, Endika
000125967 245__ $$aIdentification and characterization of miRNAs in spleens of sheep subjected to repetitive vaccination
000125967 260__ $$c2023
000125967 5060_ $$aAccess copy available to the general public$$fUnrestricted
000125967 5203_ $$aAccumulative evidence has shown that short non-coding RNAs such as miRNAs can regulate the innate and adaptive immune responses. Aluminium hydroxide is a commonly used adjuvant in human and veterinary vaccines. Despite its extended use, its mechanism of action is not fully understood and very few in vivo studies have been done to enhance understanding at the molecular level. In this work, we took advantage of a previous long-term experiment in which lambs were exposed to three different treatments by parallel subcutaneous inoculations with aluminium-containing commercial vaccines, an equivalent dose of aluminium or mock injections. Spleen samples were used for miRNA-seq. A total of 46 and 16 miRNAs were found differentially expressed when animals inoculated with commercial vaccines or the adjuvant alone were compared with control animals, respectively. Some miRNAs previously related to macrophage polarization were found dysregulated exclusively by the commercial vaccine treatment but not in the aluminium inoculated animals. The dysregulated miRNAs in vaccine group let-7b-5p, miR-29a-3p, miR-27a and miR-101-3p are candidates for further research, since they may play key roles in the immune response induced by aluminium adjuvants added to vaccines. Finally, protein–protein interaction network analysis points towards leucocyte transendothelial migration as a specific mechanism in animals receiving adjuvant only.
000125967 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2013-49137-C3-3-R
000125967 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000125967 590__ $$a3.8$$b2023
000125967 592__ $$a0.9$$b2023
000125967 591__ $$aMULTIDISCIPLINARY SCIENCES$$b25 / 134 = 0.187$$c2023$$dQ1$$eT1
000125967 593__ $$aMultidisciplinary$$c2023$$dQ1
000125967 594__ $$a7.5$$b2023
000125967 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000125967 700__ $$aBilbao-Arribas, Martin
000125967 700__ $$aAbendaño, Naiara
000125967 700__ $$0(orcid)0000-0002-6178-4801$$aAsín, Javier
000125967 700__ $$0(orcid)0000-0002-8133-2124$$aPérez, Marta$$uUniversidad de Zaragoza
000125967 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, Lluís$$uUniversidad de Zaragoza
000125967 700__ $$aJugo, Begoña M.
000125967 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000125967 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000125967 773__ $$g13 (2023), 6239 [10 pp.]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000125967 8564_ $$s3493936$$uhttps://zaguan.unizar.es/record/125967/files/texto_completo.pdf$$yVersión publicada
000125967 8564_ $$s2723844$$uhttps://zaguan.unizar.es/record/125967/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000125967 909CO $$ooai:zaguan.unizar.es:125967$$particulos$$pdriver
000125967 951__ $$a2024-11-22-12:00:53
000125967 980__ $$aARTICLE