125972 20241125101136.0 doi 10.1016/j.cimid.2023.101983 sideral 133479 ART-2023-133479 eng Millán, Javier (orcid)0000-0001-5608-781X Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile 2023 Access copy available to the general public Unrestricted Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores. info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.0 2023 VETERINARY SCIENCES 43 / 167 = 0.257 2023 Q2 T1 IMMUNOLOGY 144 / 181 = 0.796 2023 Q4 T3 MICROBIOLOGY 125 / 161 = 0.776 2023 Q4 T3 0.543 2023 Veterinary (miscellaneous) 2023 Q1 Medicine (miscellaneous) 2023 Q2 Microbiology 2023 Q3 Immunology and Allergy 2023 Q3 Immunology 2023 Q3 Infectious Diseases 2023 Q3 4.6 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sepúlveda-García, Paulina Di Cataldo, Sophia Canales, Nivia Sallaberry-Pincheira, Nicole Painean, Javier Cevidanes, Aitor Müller, Ananda 96 (2023), 101983 [9 pp.] Comp. immunol. microbiol. infect. dis. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES 0147-9571 2316959 http://zaguan.unizar.es/record/125972/files/texto_completo.pdf Versión publicada 2533885 http://zaguan.unizar.es/record/125972/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:125972 articulos driver 2024-11-22-12:01:06 ARTICLE