doi:10.3390/photonics10040467engConsejo, AlejandraBasabilbaso, SilviaRemon, LauraCorneal densitometry with Galilei dual Scheimpflug analyzerART-2023-133593This study aims to apply the densitometry distribution analysis (DDA) method to study corneal densitometry depending on age and corneal region from Galilei Dual Scheimpflug Analyzer tomography. A total of 83 healthy participants aged 39.02 ± 18.34 years (range 9–81 years) were screened using a Ziemer Galilei G2. Images were analysed using the DDA, and two parameters, α (corneal transparency) and β (corneal homogeneity), were estimated. A two-way ANOVA analysis was performed to investigate whether α and β are influenced by age, corneal región (four concentric areas were considered), and their interaction. The parameters α and β statistically change with age and corneal region. A statistically significant interaction effect of 13% (α) and 11% (β) exists between age and corneal region. However, the corneal region plays a more significant role than aging in corneal densitometry; 31% (α) and 51% (β) of the variance can be attributed to the corneal region, while 28% (α) and 5% (β) can be attributed solely to aging. Corneal densitometry can be objectively assessed from Galilei G2 images using the DDA method. The corneal region plays a more significant role than aging in corneal densitometry. Consequently, general results on corneal densitometry and aging should be taken cautiously.2023http://zaguan.unizar.es/record/12598510.3390/photonics10040467http://zaguan.unizar.es/record/125985oai:zaguan.unizar.es:125985info:eu-repo/grantAgreement/EUR/MICINN/TED2021-130723A-I00Photonics 10, 4 (2023), 467 [10 pp.]byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess