000126015 001__ 126015 000126015 005__ 20241125101151.0 000126015 0247_ $$2doi$$a10.3390/v15051107 000126015 0248_ $$2sideral$$a133579 000126015 037__ $$aART-2023-133579 000126015 041__ $$aeng 000126015 100__ $$0(orcid)0000-0002-0036-6630$$aAlgarate, Sonia$$uUniversidad de Zaragoza 000126015 245__ $$aUsefulness of non-skin samples in the PCR diagnosis of Mpox (Monkeypox) 000126015 260__ $$c2023 000126015 5060_ $$aAccess copy available to the general public$$fUnrestricted 000126015 5203_ $$aCases of mpox have been reported in several European countries, including Spain. Our objective was to evaluate the usefulness of serum and nasopharyngeal samples for diagnosis of mpox. The presence of MPXV DNA was studied using real-time PCR (CerTest Biotec, Zaragoza, Spain) in 106 samples from 50 patients: 32 skin, 31 anogenital, 25 sera, and 18 nasopharyngeal/pharyngeal, in the Hospital Clínico Universitario of Zaragoza (Spain). Sixty-three samples from twenty-seven patients were MPXV PCR-positive. The real-time PCR Ct values in the anogenital and skin samples were lower than serum and nasopharyngeal samples. More than 90% of anogenital (95.7%), serum (94.4%), and skin (92.9%) samples were real-time PCR-positive. Eighteen (66.7%) of the twenty-seven patients who were MPXV PCR-positive had antecedents or presented with one to three sexually transmitted infection (STI) agents. Our results indicate that the use of serum samples can help facilitate the diagnosis of MPXV infections. 000126015 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000126015 590__ $$a3.8$$b2023 000126015 592__ $$a1.14$$b2023 000126015 591__ $$aVIROLOGY$$b17 / 41 = 0.415$$c2023$$dQ2$$eT2 000126015 593__ $$aInfectious Diseases$$c2023$$dQ1 000126015 593__ $$aVirology$$c2023$$dQ2 000126015 594__ $$a7.3$$b2023 000126015 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000126015 700__ $$aBueno, Jessica 000126015 700__ $$0(orcid)0000-0002-5938-1982$$aCrusells, María J.$$uUniversidad de Zaragoza 000126015 700__ $$0(orcid)0000-0001-8789-6783$$aAra, Mariano$$uUniversidad de Zaragoza 000126015 700__ $$0(orcid)0000-0003-2742-8827$$aAlonso, Henar 000126015 700__ $$aAlvarado, Elena 000126015 700__ $$aDucons, María 000126015 700__ $$aArnal, Sara 000126015 700__ $$0(orcid)0000-0001-5134-1006$$aBenito, Rafael$$uUniversidad de Zaragoza 000126015 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología 000126015 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina 000126015 7102_ $$11007$$2183$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cÁrea Dermatología 000126015 773__ $$g15, 5 (2023), 1107 [7 pp.]$$pVIRUSES-BASEL$$tVIRUSES-BASEL$$x1999-4915 000126015 8564_ $$s589906$$uhttps://zaguan.unizar.es/record/126015/files/texto_completo.pdf$$yVersión publicada 000126015 8564_ $$s2560839$$uhttps://zaguan.unizar.es/record/126015/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000126015 909CO $$ooai:zaguan.unizar.es:126015$$particulos$$pdriver 000126015 951__ $$a2024-11-22-12:06:57 000126015 980__ $$aARTICLE