126225 20241125101131.0 doi 10.1039/d3lc00075c sideral 133408 ART-2023-133408 eng González-Lana, Sandra Surface modifications of COP-based microfluidic devices for improved immobilisation of hydrogel proteins: long-term 3D culture with contractile cell types and ischaemia model 2023 Access copy available to the general public Unrestricted The tissue microenvironment plays a crucial role in tissue homeostasis and disease progression. However, the in vitro simulation has been limited by the lack of adequate biomimetic models in the last decades. Thanks to the advent of microfluidic technology for cell culture applications, these complex microenvironments can be recreated by combining hydrogels, cells and microfluidic devices. Nevertheless, this advance has several limitations. When cultured in three-dimensional (3D) hydrogels inside microfluidic devices, contractile cells may exert forces that eventually collapse the 3D structure. Disrupting the compartmentalisation creates an obstacle to long-term or highly cell-concentrated assays, which are extremely relevant for multiple applications such as fibrosis or ischaemia. Therefore, we tested surface treatments on cyclic-olefin polymer-based microfluidic devices (COP-MD) to promote the immobilisation of collagen as a 3D matrix protein. Thus, we compared three surface treatments in COP devices for culturing human cardiac fibroblasts (HCF) embedded in collagen hydrogels. We determined the immobilisation efficiency of collagen hydrogel by quantifying the hydrogel transversal area within the devices at the studied time points. Altogether, our results indicated that surface modification with polyacrylic acid photografting (PAA-PG) of COP-MD is the most effective treatment to avoid the quick collapse of collagen hydrogels. As a proof-of-concept experiment, and taking advantage of the low-gas permeability properties of COP-MD, we studied the application of PAA-PG pre-treatment to generate a self-induced ischaemia model. Different necrotic core sizes were developed depending on initial HCF density seeding with no noticeable gel collapse. We conclude that PAA-PG allows long-term culture, gradient generation and necrotic core formation of contractile cell types such as myofibroblasts. This novel approach will pave the way for new relevant in vitro co-culture models where fibroblasts play a key role such as wound healing, tumour microenvironment and ischaemia within microfluidic devices. info:eu-repo/grantAgreement/ES/AEI/PID2021-126051OB-C41 info:eu-repo/grantAgreement/ES/DGA/E15-20R info:eu-repo/grantAgreement/ES/DGA/E47-20R info:eu-repo/grantAgreement/ES/DGA-FEDER/LMP221_21 info:eu-repo/grantAgreement/ES/DGA/T62-20R info:eu-repo/grantAgreement/EC/H2020/778354/EU/Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine/CISTEM This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 778354-CISTEM info:eu-repo/grantAgreement/EC/H2020/829010/EU/Advanced and versatile PRInting platform for the next generation of active Microfluidic dEvices/PRIME This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 829010-PRIME info:eu-repo/grantAgreement/ES/ISCIII/PFIS/IF16-00050 info:eu-repo/grantAgreement/ES/MINECO/DI2017-09585 info:eu-repo/grantAgreement/ES/MINECO/PID2020-118485RB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 6.1 2023 BIOCHEMICAL RESEARCH METHODS 5 / 85 = 0.059 2023 Q1 T1 CHEMISTRY, ANALYTICAL 8 / 106 = 0.075 2023 Q1 T1 INSTRUMENTS & INSTRUMENTATION 7 / 76 = 0.092 2023 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 51 / 231 = 0.221 2023 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 39 / 141 = 0.277 2023 Q2 T1 1.246 2023 Biochemistry 2023 Q1 Bioengineering 2023 Q1 Biomedical Engineering 2023 Q1 Chemistry (miscellaneous) 2023 Q1 Nanoscience and Nanotechnology 2023 Q2 11.1 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Randelovic, Teodora (orcid)0000-0001-7232-7588 Ciriza, Jesús Universidad de Zaragoza (orcid)0000-0002-8666-622X López-Valdeolivas, María Monge, Rosa Sánchez-Somolinos, Carlos (orcid)0000-0003-3900-2866 Ochoa, Ignacio Universidad de Zaragoza (orcid)0000-0003-2410-5678 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 23, 10 (2023), 2434-2446 Lab chip Lab on a chip 1473-0197 3130526 http://zaguan.unizar.es/record/126225/files/texto_completo.pdf Versión publicada 2510189 http://zaguan.unizar.es/record/126225/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:126225 articulos driver 2024-11-22-11:59:06 ARTICLE