000126238 001__ 126238
000126238 005__ 20241125101136.0
000126238 0247_ $$2doi$$a10.1016/j.scienta.2023.112087
000126238 0248_ $$2sideral$$a133646
000126238 037__ $$aART-2023-133646
000126238 041__ $$aeng
000126238 100__ $$aTedesco, Sara
000126238 245__ $$aEarly detection of grapevine graft incompatibility: insights into translocated and virus-induced incompatibility
000126238 260__ $$c2023
000126238 5060_ $$aAccess copy available to the general public$$fUnrestricted
000126238 5203_ $$aIn vineyards to control phylloxera (Daktulosphaira vitifolia Ficth) attacks in Vitis vinifera L., heterografted vines are planted using American vines hybrids as rootstocks. However, graft incompatibilities can affect grape yield and plant longevity. Thus, to identify early graft incompatibility factors, we established in vitro micrografting protocols coupled with histology and histochemistry analysis in grapevine graft combinations of known compatibility behavior. The histochemical characterization of the graft union revealed irregular cell arrangement, slower vascular differentiation, persistence of the necrotic layer, accumulation of starch, and lower differentiation of phloem cells in hetero- compared to homografts, indicating the presence of translocated incompatibility symptoms. We highlight the utility of evaluating the graft interface cellular arrangement and starch content via calcofluor and I2KI staining, respectively, as allowed to identify the graft combinations with lower graft success. Wounded and grafted Syrah plantlets pointed out an impaired sucrose distribution in these plants and levels of Grapevine Rupestris Stem Pitting associated Virus (GRSPaV) infections correlated with graft (un)-success in two Syrah clones micrografted onto 110-Ritcher rootstock. Furthermore, silencing GRSPaV before grafting increased graft success rates. We propose that grapevine graft incompatibility is mainly a virus-induced phenomenon that can arise even in certified plants.
000126238 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A12
000126238 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000126238 590__ $$a3.9$$b2023
000126238 592__ $$a0.833$$b2023
000126238 591__ $$aHORTICULTURE$$b6 / 38 = 0.158$$c2023$$dQ1$$eT1
000126238 593__ $$aHorticulture$$c2023$$dQ1
000126238 594__ $$a8.6$$b2023
000126238 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000126238 700__ $$aIrisarri, Patricia
000126238 700__ $$aTeixeira Santos, Margarida
000126238 700__ $$aFevereiro, Pedro
000126238 700__ $$0(orcid)0000-0002-4988-9467$$aPina, Ana
000126238 700__ $$aKragler, Friedrich
000126238 773__ $$g318 (2023), 112087 [11 pp.]$$pSci. hortic.$$tSCIENTIA HORTICULTURAE$$x0304-4238
000126238 8564_ $$s11736457$$uhttps://zaguan.unizar.es/record/126238/files/texto_completo.pdf$$yVersión publicada
000126238 8564_ $$s2493256$$uhttps://zaguan.unizar.es/record/126238/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000126238 909CO $$ooai:zaguan.unizar.es:126238$$particulos$$pdriver
000126238 951__ $$a2024-11-22-12:00:57
000126238 980__ $$aARTICLE