000126273 001__ 126273
000126273 005__ 20241125101149.0
000126273 0247_ $$2doi$$a10.1016/j.fm.2023.104285
000126273 0248_ $$2sideral$$a133668
000126273 037__ $$aART-2023-133668
000126273 041__ $$aeng
000126273 100__ $$0(orcid)0000-0002-4027-5637$$aGuillén, S.
000126273 245__ $$aGenotypic and phenotypic characterization of a Salmonella Typhimurium strain resistant to pulsed electric fields
000126273 260__ $$c2023
000126273 5060_ $$aAccess copy available to the general public$$fUnrestricted
000126273 5203_ $$aPulsed Electric Fields (PEF) technology is regarded as one of the most interesting alternatives to current food preservation methods, due to its capability to inactivate vegetative microorganisms while leaving the product's organoleptic and nutritional properties mostly unchanged. However, many aspects regarding the mechanisms of bacterial inactivation by PEF are still not fully understood. The aim of this study was to obtain further insight into the mechanisms responsible for the increased resistance to PEF of a Salmonella Typhimurium SL1344 variant (SL1344-RS, Sagarzazu et al., 2013), and to quantify the impact that the acquisition of PEF resistance has on other aspects of S. enterica physiology, such as growth fitness, biofilm formation ability, virulence and antibiotic resistance. WGS, RNAseq and qRT-PCR assays indicated that the increased PEF resistance of the SL1344-RS variant is due to a higher RpoS activity caused by a mutation in the hnr gene. This increased RpoS activity also results in higher resistance to multiple stresses (acidic, osmotic, oxidative, ethanol and UV-C, but not to heat and HHP), decreased growth rate in M9-Gluconate (but not in TSB-YE or LB-DPY), increased ability to adhere to Caco-2 cells (but no significant change in invasiveness) and enhanced antibiotic resistance (to six out of eight agents). This study significantly contributes to the understanding of the mechanisms of the development of stress resistance in Salmonellae and underscores the crucial role played by RpoS in this process. Further studies are needed to determine whether this PEF-resistant variant would represent a higher, equal or lower associated hazard than the parental strain.
000126273 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-84084-R
000126273 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000126273 590__ $$a4.5$$b2023
000126273 592__ $$a1.026$$b2023
000126273 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b34 / 174 = 0.195$$c2023$$dQ1$$eT1
000126273 593__ $$aFood Science$$c2023$$dQ1
000126273 591__ $$aMICROBIOLOGY$$b40 / 161 = 0.248$$c2023$$dQ1$$eT1
000126273 593__ $$aMicrobiology$$c2023$$dQ2
000126273 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b42 / 173 = 0.243$$c2023$$dQ1$$eT1
000126273 594__ $$a11.3$$b2023
000126273 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000126273 700__ $$aNadal, L.$$uUniversidad de Zaragoza
000126273 700__ $$0(orcid)0000-0002-4121-8087$$aHalaihel, N.$$uUniversidad de Zaragoza
000126273 700__ $$0(orcid)0000-0002-7971-4828$$aMañas, P.$$uUniversidad de Zaragoza
000126273 700__ $$0(orcid)0000-0002-5049-3646$$aCebrián, G.$$uUniversidad de Zaragoza
000126273 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000126273 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000126273 773__ $$g113 (2023), 104285 [9 pp.]$$pFood microbiol.$$tFOOD MICROBIOLOGY$$x0740-0020
000126273 8564_ $$s766516$$uhttps://zaguan.unizar.es/record/126273/files/texto_completo.pdf$$yVersión publicada
000126273 8564_ $$s2489321$$uhttps://zaguan.unizar.es/record/126273/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000126273 909CO $$ooai:zaguan.unizar.es:126273$$particulos$$pdriver
000126273 951__ $$a2024-11-22-12:06:06
000126273 980__ $$aARTICLE