126342 20241125101138.0 doi 10.1007/s11259-023-10087-0 sideral 133807 ART-2023-133807 eng Villanueva-Saz, Sergio Universidad de Zaragoza (orcid)0000-0001-6209-4282 The dynamics of neutralizing antibodies against SARS-CoV-2 in cats naturally exposed to virus reveals an increase in antibody activity after re-infection 2023 Access copy available to the general public Unrestricted Severe Acute Respiratory Syndrome Coronavirus 2 is the causative agent of Coronavirus Disease 2019 in humans. To date, little is known about the persistence of antibodies against SARS-CoV-2 in animals under natural conditions, in particular susceptible pets such as cat. This study reports the detection and monitoring of the humoral response against SARS-CoV-2 including the detection of immunoglobulins G specific for receptor binding domain of SARS-CoV-2 spike protein by an enzyme-linked immunosorbent assay and neutralizing antibodies by virus neutralization assay. Results showed that these antibodies last longer than 16 months in two naturally apparently healthy infected cats with the absence of clinicopathological findings during the follow-up. Moreover, re-infection is also possible with an important increase in virus neutralization test titers in both animals with no evident systemic signs found during each physical examination and with values of hematologic and biochemical parameters inside the normal reference intervals. Our results confirm a slow but progressive decrease of the kinetics and immunity of neutralizing antibodies in cats after the infection. Furthermore, similar to humans SARS-CoV-2 reinfection can stimulate an increase of the neutralizing antibodies determined by these two serological techniques in domestic cats. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 1.8 2023 VETERINARY SCIENCES 53 / 167 = 0.317 2023 Q2 T1 0.483 2023 Veterinary (miscellaneous) 2023 Q2 Medicine (miscellaneous) 2023 Q3 2.5 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Martínez, Marivi (orcid)0000-0002-9071-1919 Rueda, Pablo Bolea, Sara Pérez, María Dolores Universidad de Zaragoza (orcid)0000-0003-2555-8425 Verde, Maite Universidad de Zaragoza (orcid)0000-0003-2957-1379 Yzuel, Andrés Hurtado-Guerrero, Ramón (orcid)0000-0002-3122-9401 Pardo, Julián Universidad de Zaragoza (orcid)0000-0003-0154-0730 Santiago, Llipsy (orcid)0000-0002-1861-5981 Fernández, Antonio Universidad de Zaragoza (orcid)0000-0002-2557-4890 Arias, Maykel (orcid)0000-0002-9730-2210 1011 566 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Inmunología 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 47 (2023), 2179–2184 Vet. res. commun. Veterinary Research Communications 0165-7380 773356 http://zaguan.unizar.es/record/126342/files/texto_completo.pdf Versión publicada 2083915 http://zaguan.unizar.es/record/126342/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:126342 articulos driver 2024-11-22-12:01:35 ARTICLE