000126359 001__ 126359
000126359 005__ 20240720100850.0
000126359 0247_ $$2doi$$a10.3389/fbioe.2023.1197710
000126359 0248_ $$2sideral$$a133757
000126359 037__ $$aART-2023-133757
000126359 041__ $$aeng
000126359 100__ $$aBerzosa, Alejandro$$uUniversidad de Zaragoza
000126359 245__ $$aSequential extraction of compounds of interest from yeast biomass assisted by pulsed electric fields
000126359 260__ $$c2023
000126359 5060_ $$aAccess copy available to the general public$$fUnrestricted
000126359 5203_ $$aOne strategy to reduce cost and improve feasibility of waste-yeast biomass valorization is to obtain a spectrum of marketable products rather than just a single one. This study explores the potential of Pulsed Electric Fields (PEF) for the development of a cascade process designed to obtain several valuable products from Saccharomyces cerevisiae yeast biomass. Yeast biomass was treated by PEF, which affected the viability of 50%, 90%, and over 99% of S. cerevisiae cells, depending on treatment intensity. Electroporation caused by PEF allowed access to the cytoplasm of the yeast cell without causing total breakdown of the cell structure. This outcome was an essential prerequisite to be able to perform a sequential extraction of several value-added biomolecules from yeast cells located in the cytosol and in the cell wall. After incubating yeast biomass previously subjected to a PEF treatment that affected the viability of 90% of cells for 24 h, an extract with 114.91 ± 2.86, 7.08 ± 0.64, and 187.82 ± 3.75 mg/g dry weight of amino acids, glutathione, and protein, respectively, was obtained. In a second step, the extract rich in cytosol components was removed after 24 h of incubation and the remaining cell biomass was re-suspended with the aim of inducing cell wall autolysis processes triggered by the PEF treatment. After 11 days of incubation, a soluble extract containing mannoproteins and pellets rich in β-glucans were obtained. In conclusion, this study proved that electroporation triggered by PEF permitted the development of a cascade procedure designed to obtain a spectrum of valuable biomolecules from S. cerevisiae yeast biomass while reducing the generation of waste.
000126359 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2020-113620RB-100
000126359 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000126359 590__ $$a4.3$$b2023
000126359 592__ $$a0.893$$b2023
000126359 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b41 / 174 = 0.236$$c2023$$dQ1$$eT1
000126359 593__ $$aBiomedical Engineering$$c2023$$dQ1
000126359 591__ $$aENGINEERING, BIOMEDICAL$$b39 / 122 = 0.32$$c2023$$dQ2$$eT1
000126359 593__ $$aHistology$$c2023$$dQ2
000126359 593__ $$aBiotechnology$$c2023$$dQ2
000126359 593__ $$aBioengineering$$c2023$$dQ2
000126359 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000126359 700__ $$0(orcid)0000-0003-4744-8649$$aDelso, Carlota$$uUniversidad de Zaragoza
000126359 700__ $$aSanz, Jorge$$uUniversidad de Zaragoza
000126359 700__ $$0(orcid)0000-0002-5086-7839$$aSánchez-Gimeno, Cristina$$uUniversidad de Zaragoza
000126359 700__ $$0(orcid)0000-0003-3957-9091$$aRaso, Javier$$uUniversidad de Zaragoza
000126359 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000126359 773__ $$g11 (2023), 1197710. [12 pp.]$$pFront. Bioeng. Biotechnol.$$tFrontiers in Bioengineering and Biotechnology$$x2296-4185
000126359 8564_ $$s1941989$$uhttps://zaguan.unizar.es/record/126359/files/texto_completo.pdf$$yVersión publicada
000126359 8564_ $$s2144846$$uhttps://zaguan.unizar.es/record/126359/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000126359 909CO $$ooai:zaguan.unizar.es:126359$$particulos$$pdriver
000126359 951__ $$a2024-07-19-18:49:03
000126359 980__ $$aARTICLE