000126419 001__ 126419
000126419 005__ 20241125101137.0
000126419 0247_ $$2doi$$a10.1016/j.animal.2023.100789
000126419 0248_ $$2sideral$$a133480
000126419 037__ $$aART-2023-133480
000126419 041__ $$aeng
000126419 100__ $$aRomero, P.
000126419 245__ $$aEvaluating the effect of phenolic compounds as hydrogen acceptors when ruminal methanogenesis is inhibited in vitro – Part 2. Dairy goats
000126419 260__ $$c2023
000126419 5060_ $$aAccess copy available to the general public$$fUnrestricted
000126419 5203_ $$aMost mitigation strategies to reduce enteric methane (CH4) production in the rumen induce an excess of rumen dihydrogen (H2) that is expelled and consequently not redirected to the synthesis of metabolites that can be utilised by the ruminant. We hypothesised that phenolic compounds can be potential H2 acceptors when added to the diet, as they can be degraded to compounds that may be beneficial for the animal, using part of the H2 available when ruminal methanogenesis is inhibited. We performed four in vitro incubation experiments using rumen inoculum from Murciano-Granadina adult goats: Experiment 1 examined the inhibitory potential of Asparagopsis taxiformis (AT) at different concentrations (0, 1, 2, 3, 4 and 5% of the substrate on a DM basis) in 24 h incubations; Experiment 2 investigated the effect of a wide range of phenolic compounds (phenol, catechol, resorcinol, hydroquinone, pyrogallol, phloroglucinol, gallic acid and formic acid) at different doses (0, 2, 4, and 6 mM) on rumen fermentation for 24 h; Experiment 3 evaluated the combined effect of each phenolic compound at 6 mM with AT at 2% DM in sequential batch cultures for 5 days; and Experiment 4 examined the dose–response effect of phloroglucinol at different concentrations (0, 6, 16, 26 and 36 mM) combined with AT in sequential batch cultures for 5 days. Results from Experiment 1 confirmed that AT at 2% DM substantially inhibited CH4 production while significantly increasing H2 accumulation and decreasing the acetate:propionate ratio. Results from Experiment 2 showed that phenolic compounds did not negatively affect rumen fermentation at any dose. In Experiment 3, each phenolic compound at 6 mM combined with AT at 2% DM inhibited CH4 production. Phloroglucinol numerically decreased H2 accumulation and significantly increased total gas production (TGP), volatile fatty acid (VFA) production and the acetate:propionate ratio. In Experiment 4, phloroglucinol at increasing doses supplemented with AT at 2% DM significantly decreased H2 accumulation and the abundances of archaea, protozoa and fungi abundances, and increased TGP, total VFA production and the acetate:propionate ratio in a dose-dependent way. In conclusion, combined treatment with AT and phloroglucinol was successful to mitigate CH4 production while preventing the accumulation of H2, leading to an increase in acetate and total VFA production and therefore an improvement in rumen fermentation in goats.
000126419 536__ $$9info:eu-repo/grantAgreement/EC/H2020/818368/EU/Microbiome Applications for Sustainable food systems through Technologies and EnteRprise/MASTER$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 818368-MASTER$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2019-027764-I/AEI/10.13039/501100011033
000126419 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000126419 590__ $$a4.0$$b2023
000126419 592__ $$a1.01$$b2023
000126419 591__ $$aVETERINARY SCIENCES$$b8 / 167 = 0.048$$c2023$$dQ1$$eT1
000126419 593__ $$aAnimal Science and Zoology$$c2023$$dQ1
000126419 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b4 / 80 = 0.05$$c2023$$dQ1$$eT1
000126419 594__ $$a7.5$$b2023
000126419 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000126419 700__ $$aHuang, R.
000126419 700__ $$aJiménez, E.
000126419 700__ $$aPalma-Hidalgo, J. M.
000126419 700__ $$aUngerfeld, E. M.
000126419 700__ $$aPopova, M.
000126419 700__ $$aMorgavi, D. P.
000126419 700__ $$0(orcid)0000-0001-5880-6021$$aBelanche, A.$$uUniversidad de Zaragoza
000126419 700__ $$aYáñez-Ruiz, D. R.
000126419 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000126419 773__ $$g17, 5 (2023), 100789 [11 pp.]$$pAnimal$$tANIMAL$$x1751-7311
000126419 8564_ $$s517842$$uhttps://zaguan.unizar.es/record/126419/files/texto_completo.pdf$$yVersión publicada
000126419 8564_ $$s2664885$$uhttps://zaguan.unizar.es/record/126419/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000126419 909CO $$ooai:zaguan.unizar.es:126419$$particulos$$pdriver
000126419 951__ $$a2024-11-22-12:01:18
000126419 980__ $$aARTICLE