000126432 001__ 126432 000126432 005__ 20240731103349.0 000126432 0247_ $$2doi$$a10.1016/j.rvsc.2023.04.020 000126432 0248_ $$2sideral$$a133870 000126432 037__ $$aART-2023-133870 000126432 041__ $$aeng 000126432 100__ $$aDashti, A. 000126432 245__ $$aOccurrence and limited zoonotic potential of Cryptosporidium spp., Giardia duodenalis, and Balantioides coli infections in free-ranging and farmed wild ungulates in Spain 000126432 260__ $$c2023 000126432 5060_ $$aAccess copy available to the general public$$fUnrestricted 000126432 5203_ $$aLittle information is currently available on the occurrence and molecular diversity of the enteric protozoan parasites Cryptosporidium spp., Giardia duodenalis, and Balantioides coli in wild ungulates and the role of these host species as potential sources of environmental contamination and consequent human infections. The presence of these three pathogens was investigated in eight wild ungulate species present in Spain (genera Ammotragus, Capra, Capreolus, Cervus, Dama, Ovis, Rupicapra, and Sus) by molecular methods. Faecal samples were retrospectively collected from free-ranging (n = 1058) and farmed (n = 324) wild ungulates from the five Spanish bioregions. Overall infection rates were 3.0% (42/1382; 95% CI: 2.1–3.9%) for Cryptosporidium spp., 5.4% (74/1382; 95% CI: 4.2–6.5%) for G. duodenalis, and 0.7% (9/1382; 95% CI: 0.3–1.2%) for B. coli. Cryptosporidium infection was detected in roe deer (7.5%), wild boar (7.0%) and red deer (1.5%), and G. duodenalis in southern chamois (12.9%), mouflon (10.0%), Iberian wild goat (9.0%), roe deer (7.5%), wild boar (5.6%), fallow deer (5.2%) and red deer (3.8%). Balantioides coli was only detected in wild boar (2.5%, 9/359). Sequence analyses revealed the presence of six distinct Cryptosporidium species: C. ryanae in red deer, roe deer, and wild boar; C. parvum in red deer and wild boar; C. ubiquitum in roe deer; C. scrofarum in wild boar; C. canis in roe deer; and C. suis in red deer. Zoonotic assemblages A and B were detected in wild boar and red deer, respectively. Ungulate-adapted assemblage E was identified in mouflon, red deer, and southern chamois. Attempts to genotype samples positive for B. coli failed. Sporadic infections by canine- or swine-adapted species may be indicative of potential cross-species transmission, although spurious infections cannot be ruled out. Molecular evidence gathered is consistent with parasite mild infections and limited environmental contamination with (oo)cysts. Free-ranging wild ungulate species would not presumably play a significant role as source of human infections by these pathogens. Wild ruminants do not seem to be susceptible hosts for B. coli. 000126432 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/CD19-00011$$9info:eu-repo/grantAgreement/ES/MINECO/CGL2016-80543-P$$9info:eu-repo/grantAgreement/ES/ISCIII/FI20-00002$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI19-00029$$9info:eu-repo/grantAgreement/ES/MCIU/IJC-2020-042629-I$$9info:eu-repo/grantAgreement/ES/MINECO/CGL2012-40043-C02-01$$9info:eu-repo/grantAgreement/ES/MINECO/CGL2012-40043-C02-02$$9info:eu-repo/grantAgreement/ES/MINECO/PID2020-115046GB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2016-2020 000126432 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000126432 590__ $$a2.2$$b2023 000126432 592__ $$a0.617$$b2023 000126432 591__ $$aVETERINARY SCIENCES$$b32 / 167 = 0.192$$c2023$$dQ1$$eT1 000126432 593__ $$aVeterinary (miscellaneous)$$c2023$$dQ1 000126432 594__ $$a4.4$$b2023 000126432 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000126432 700__ $$aKöster, P. C. 000126432 700__ $$aBailo, B. 000126432 700__ $$aSánchez de las Matas, A. 000126432 700__ $$aHabela, M. Á. 000126432 700__ $$aRivero-Juarez, A. 000126432 700__ $$aVicente, J. 000126432 700__ $$aSerrano, E. 000126432 700__ $$0(orcid)0000-0002-6770-3400$$aArnal, M. C.$$uUniversidad de Zaragoza 000126432 700__ $$0(orcid)0000-0003-3289-4267$$aFernández de Luco, D.$$uUniversidad de Zaragoza 000126432 700__ $$aMorrondo, P. 000126432 700__ $$aArmenteros, J. A. 000126432 700__ $$aBalseiro, A. 000126432 700__ $$aCardona, G. A. 000126432 700__ $$aMartínez-Carrasco, C. 000126432 700__ $$aOrtiz, J. A. 000126432 700__ $$aCarpio, A. J. 000126432 700__ $$aCalero-Bernal, R. 000126432 700__ $$aGonzález-Barrio, D. 000126432 700__ $$aCarmena, D. 000126432 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000126432 773__ $$g159 (2023), 189-197$$pRes. Vet. Sci.$$tRESEARCH IN VETERINARY SCIENCE$$x0034-5288 000126432 8564_ $$s402668$$uhttps://zaguan.unizar.es/record/126432/files/texto_completo.pdf$$yPostprint 000126432 8564_ $$s1842309$$uhttps://zaguan.unizar.es/record/126432/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000126432 909CO $$ooai:zaguan.unizar.es:126432$$particulos$$pdriver 000126432 951__ $$a2024-07-31-09:53:48 000126432 980__ $$aARTICLE