000126434 001__ 126434
000126434 005__ 20241125101146.0
000126434 0247_ $$2doi$$a10.52312/jdrs.2023.892
000126434 0248_ $$2sideral$$a133897
000126434 037__ $$aART-2023-133897
000126434 041__ $$aeng
000126434 100__ $$aCalvo, Jorge$$uUniversidad de Zaragoza
000126434 245__ $$aGranzyme-A deficiency attenuates experimental osteoarthritis in mice, but perforin deficiency does not
000126434 260__ $$c2023
000126434 5060_ $$aAccess copy available to the general public$$fUnrestricted
000126434 5203_ $$aObjectives: This study aims to assess the development of osteoarthritis (OA) in granzyme A- (gzmA) and B- (gzmB) and perforin- (perf) knockout mice.
Materials and methods: A total of 75 male and female C57BL/6 (eight to nine-week-old) mice were allocated to: gzmA-deficient (gzmA-/-) (11 females, 8 males), gzmB-deficient (gzmB-/-) (9 females, 8 males), perf-deficient (perf-/-) (10 females, 9 males), and control group (10 females, 10 males). Osteoarthritis was induced in the right knee by instability of the meniscus medial ligament. Sham surgery was practiced in the left knee. Knee samples obtained eight weeks after surgery were stained (Safranin-O) and blindly scored in lateral and medial femur and tibia using the Osteoarthritis Research Society International scale (OARSI) (from Grade 0, cartilage intact to 6, deformation), (five stages from 0, no OA to 4, >50% surface involvement); OARSI score (grade x stage); and a semi-quantitative scale from Grade 0 (normal) to 6 (cartilage erosion >80%).
Results: Significantly higher values in all scales in the right knees compared to the left knees in male and female mice were observed (p<0.05). Males of all strains showed in the right knee higher values than females on all scales. Deficiency of perforin did not modify OA severity in any sex. The gzmA-/- females presented less degenerative changes than the other groups.
Conclusion: Our study results show that sex plays an important role in the development of experimental OA in mice. Deficiency of gzmA can protect from the development of OA in female mice.
000126434 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/B29-17R
000126434 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000126434 590__ $$a1.9$$b2023
000126434 592__ $$a0.607$$b2023
000126434 591__ $$aSURGERY$$b119 / 292 = 0.408$$c2023$$dQ2$$eT2
000126434 593__ $$aOrthopedics and Sports Medicine$$c2023$$dQ2
000126434 591__ $$aORTHOPEDICS$$b55 / 136 = 0.404$$c2023$$dQ2$$eT2
000126434 593__ $$aSurgery$$c2023$$dQ2
000126434 593__ $$aRehabilitation$$c2023$$dQ2
000126434 594__ $$a2.5$$b2023
000126434 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000126434 700__ $$0(orcid)0000-0002-1861-5981$$aSantiago, Llipsy
000126434 700__ $$0(orcid)0000-0002-9730-2210$$aArias, Maykel
000126434 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, Julián$$uUniversidad de Zaragoza
000126434 700__ $$0(orcid)0000-0003-4489-3130$$aAlbareda, Jorge$$uUniversidad de Zaragoza
000126434 700__ $$0(orcid)0000-0003-3043-147X$$aMartínez-Lostao, Luis$$uUniversidad de Zaragoza
000126434 700__ $$0(orcid)0000-0003-2587-7506$$aGarcía-Alvarez, Felícito$$uUniversidad de Zaragoza
000126434 7102_ $$11013$$2830$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Traumatología y Ortopedia
000126434 7102_ $$11011$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Inmunología
000126434 773__ $$g34, 2 (2023), 271-278$$pJt. dis. relat. surg.$$tJoint diseases and related surgery$$x2687-4784
000126434 8564_ $$s829170$$uhttps://zaguan.unizar.es/record/126434/files/texto_completo.pdf$$yVersión publicada
000126434 8564_ $$s2672132$$uhttps://zaguan.unizar.es/record/126434/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000126434 909CO $$ooai:zaguan.unizar.es:126434$$particulos$$pdriver
000126434 951__ $$a2024-11-22-12:04:41
000126434 980__ $$aARTICLE