000126444 001__ 126444
000126444 005__ 20241125101149.0
000126444 0247_ $$2doi$$a10.1016/j.animal.2023.100788
000126444 0248_ $$2sideral$$a133823
000126444 037__ $$aART-2023-133823
000126444 041__ $$aeng
000126444 100__ $$aHuang, R.
000126444 245__ $$aEvaluating the effect of phenolic compounds as hydrogen acceptors when ruminal methanogenesis is inhibited in vitro – Part 1. Dairy cows
000126444 260__ $$c2023
000126444 5060_ $$aAccess copy available to the general public$$fUnrestricted
000126444 5203_ $$aSome antimethanogenic feed additives for ruminants promote rumen dihydrogen (H2) accumulation potentially affecting the optimal fermentation of diets. We hypothesised that combining an H2 acceptor with a methanogenesis inhibitor can decrease rumen H2 build-up and improve the production of metabolites that can be useful for the host ruminant. We performed three in vitro incubation experiments using rumen fluid from lactating Holstein cows: Experiment 1 examined the effect of phenolic compounds (phenol, catechol, resorcinol, hydroquinone, pyrogallol, phloroglucinol, and gallic acid) at 0, 2, 4, and 6 mM on ruminal fermentation for 24 h; Experiment 2 examined the combined effect of each phenolic compound from Experiment 1 at 6 mM with two different methanogenesis inhibitors (Asparagopsis taxiformis or 2-bromoethanesulfonate (BES)) for 24 h incubation; Experiment 3 examined the effect of a selected phenolic compound, phloroglucinol, with or without BES over a longer term using sequential incubations for seven days. Results from Experiment 1 showed that phenolic compounds, independently of the dose, did not negatively affect rumen fermentation, whereas results from Experiment 2 showed that phenolic compounds did not decrease H2 accumulation or modify CH4 production when methanogenesis was decreased by up to 75% by inhibitors. In Experiment 3, after three sequential incubations, phloroglucinol combined with BES decreased H2 accumulation by 72% and further inhibited CH4 production, compared to BES alone. Interestingly, supplementation with phloroglucinol (alone or in combination with the CH4 inhibitor) decreased CH4 production by 99% and the abundance of methanogenic archaea, with just a nominal increase in H2 accumulation. Supplementation of phloroglucinol also increased total volatile fatty acid (VFA), acetate, butyrate, and total gas production, and decreased ammonia concentration. This study indicates that some phenolic compounds, particularly phloroglucinol, which are naturally found in plants, could improve VFA production, decrease H2 accumulation and synergistically decrease CH4 production in the presence of antimethanogenic compounds.
000126444 536__ $$9info:eu-repo/grantAgreement/EC/H2020/818368/EU/Microbiome Applications for Sustainable food systems through Technologies and EnteRprise/MASTER$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 818368-MASTER$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2019-027764-I/AEI/10.13039/501100011033
000126444 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000126444 590__ $$a4.0$$b2023
000126444 592__ $$a1.01$$b2023
000126444 591__ $$aVETERINARY SCIENCES$$b8 / 167 = 0.048$$c2023$$dQ1$$eT1
000126444 593__ $$aAnimal Science and Zoology$$c2023$$dQ1
000126444 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b4 / 80 = 0.05$$c2023$$dQ1$$eT1
000126444 594__ $$a7.5$$b2023
000126444 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000126444 700__ $$aRomero, P.
000126444 700__ $$0(orcid)0000-0001-5880-6021$$aBelanche, A.$$uUniversidad de Zaragoza
000126444 700__ $$aUngerfeld, E. M.
000126444 700__ $$aYanez-Ruiz, D.
000126444 700__ $$aMorgavi, D. P.
000126444 700__ $$aPopova, M.
000126444 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000126444 773__ $$g17, 5 (2023), 100788 [9 pp.]$$pAnimal$$tANIMAL$$x1751-7311
000126444 8564_ $$s645719$$uhttps://zaguan.unizar.es/record/126444/files/texto_completo.pdf$$yVersión publicada
000126444 8564_ $$s2663744$$uhttps://zaguan.unizar.es/record/126444/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000126444 909CO $$ooai:zaguan.unizar.es:126444$$particulos$$pdriver
000126444 951__ $$a2024-11-22-12:06:09
000126444 980__ $$aARTICLE