126455 20241125101151.0 doi 10.3390/foods12112108 sideral 133828 ART-2023-133828 eng Ben Miri, Yamina Antifungal activity of menthol, eugenol and their combination against Aspergillus ochraceus and Aspergillus niger in vitro and in stored cereals 2023 Access copy available to the general public Unrestricted Aspergillus ochraceus and Aspergillus niger are spoilage and mycotoxin-producing fungi that can contaminate agricultural commodities and derived products. In the present study, menthol, eugenol, and their combination (mix 1:1) were tested to determine their contact and fumigation toxicity against the two fungi. Menthol, eugenol, and their mixture significantly reduced mycelial growth and spore germination at concentrations from 300 to 600 µg/mL, and the inhibitory effects showed clear dose dependence. The minimum inhibitory concentration (MIC) values against A. ochraceus were 500 µg/mL (menthol), 400 µg/mL (eugenol), and 300 µg/mL (mix 1:1), while the MIC values for A. niger were 500 µg/mL (menthol), 600 µg/mL (eugenol), and 400 µg/mL (mix 1:1). Additionally, the analyzed compounds exhibited more than 50% protection against A. ochraceus and A. niger by fumigation of stored cereal grains (maize, barley, and rice) in sealed containers. The binary mixture of menthol and eugenol showed synergistic effects against both fungi in both in vitro direct contact and stored grain fumigation trials. The results of the present study provide a scientific basis for the application of a combination of natural antifungals in food preservation. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.7 2023 FOOD SCIENCE & TECHNOLOGY 38 / 173 = 0.22 2023 Q1 T1 0.87 2023 Food Science 2023 Q1 Health (social science) 2023 Q1 Plant Science 2023 Q1 Health Professions (miscellaneous) 2023 Q1 Microbiology 2023 Q2 7.4 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Nouasri, Ahmed Herrera, Marta Universidad de Zaragoza (orcid)0000-0002-2469-0363 Djenane, Djamel Ariño, Agustín Universidad de Zaragoza (orcid)0000-0001-6325-7100 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 12, 11 (2023), 2108 [10 pp.] Foods Foods 2304-8158 760017 http://zaguan.unizar.es/record/126455/files/texto_completo.pdf Versión publicada 2758825 http://zaguan.unizar.es/record/126455/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:126455 articulos driver 2024-11-22-12:07:00 ARTICLE