000126481 001__ 126481 000126481 005__ 20241125101201.0 000126481 0247_ $$2doi$$a10.3390/cancers15092479 000126481 0248_ $$2sideral$$a133812 000126481 037__ $$aART-2023-133812 000126481 041__ $$aeng 000126481 100__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, María$$uUniversidad de Zaragoza 000126481 245__ $$aX-ray Photoelectron Spectroscopy (XPS) analysis of nitrogen environment in small extracellular vesicle membranes: a potential novel technique with application for cancer screening 000126481 260__ $$c2023 000126481 5060_ $$aAccess copy available to the general public$$fUnrestricted 000126481 5203_ $$aSmall extracellular vesicle (EV) membranes display characteristic protein-lipidic composition features that are related to their cell of origin, providing valuable clues regarding their parental cell composition and real-time state. This could be especially interesting in the case of cancer cell-derived EVs, as their membranes could serve as valuable tools in liquid biopsy applications and to detect changes in the tumor malignancy. X-Ray Photoelectron Spectroscopy (XPS) is a powerful surface analysis technique able to detect every chemical element present, being also sensitive to their chemical environment. Here we explore the use of XPS as a fast technique to characterize EV membrane composition, with possible application in cancer research. Notably, we have focused on the nitrogen environment as an indicator of the relative abundance of pyridine-type bonding, primary, secondary and tertiary amines. Specifically, we have analyzed how tumoral and healthy cells have different nitrogen chemical environments that can indicate the presence or absence of malignancy. In addition, a collection of human serum samples from cancer patients and healthy donors was also analyzed. The differential XPS analysis of EVs collected from patients confirmed that the patterns of amine evolution could be related to markers of cancer disease, opening the possibility of their use as a non-invasive blood biomarker. 000126481 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B054-12$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/Una manera de hacer Europa$$9info:eu-repo/grantAgreement/ES/DGA-FSE/Building Europe from Aragon$$9info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE$$9info:eu-repo/grantAgreement/ES/ISCIII DTS21-00130$$9info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007 000126481 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000126481 590__ $$a4.5$$b2023 000126481 592__ $$a1.391$$b2023 000126481 591__ $$aONCOLOGY$$b78 / 322 = 0.242$$c2023$$dQ1$$eT1 000126481 593__ $$aOncology$$c2023$$dQ1 000126481 593__ $$aCancer Research$$c2023$$dQ2 000126481 594__ $$a8.0$$b2023 000126481 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000126481 700__ $$aMartín-Pardillos, Ana$$uUniversidad de Zaragoza 000126481 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, Silvia$$uUniversidad de Zaragoza 000126481 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, Víctor$$uUniversidad de Zaragoza 000126481 700__ $$aCebolla, Vicente Luis 000126481 700__ $$aPazo-Cid, Roberto 000126481 700__ $$0(orcid)0000-0003-4160-9720$$aMartín-Duque, Pilar$$uUniversidad de Zaragoza 000126481 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaría, Jesús$$uUniversidad de Zaragoza 000126481 7102_ $$11013$$2275$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Estomatología 000126481 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química 000126481 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente 000126481 773__ $$g15, 9 (2023), 2479 [18 pp.]$$pCancers$$tCancers$$x2072-6694 000126481 8564_ $$s3728735$$uhttps://zaguan.unizar.es/record/126481/files/texto_completo.pdf$$yVersión publicada 000126481 8564_ $$s2731911$$uhttps://zaguan.unizar.es/record/126481/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000126481 909CO $$ooai:zaguan.unizar.es:126481$$particulos$$pdriver 000126481 951__ $$a2024-11-22-12:11:53 000126481 980__ $$aARTICLE