126481 20241125101201.0 doi 10.3390/cancers15092479 sideral 133812 ART-2023-133812 eng Sancho-Albero, María Universidad de Zaragoza (orcid)0000-0001-8762-5457 X-ray Photoelectron Spectroscopy (XPS) analysis of nitrogen environment in small extracellular vesicle membranes: a potential novel technique with application for cancer screening 2023 Access copy available to the general public Unrestricted Small extracellular vesicle (EV) membranes display characteristic protein-lipidic composition features that are related to their cell of origin, providing valuable clues regarding their parental cell composition and real-time state. This could be especially interesting in the case of cancer cell-derived EVs, as their membranes could serve as valuable tools in liquid biopsy applications and to detect changes in the tumor malignancy. X-Ray Photoelectron Spectroscopy (XPS) is a powerful surface analysis technique able to detect every chemical element present, being also sensitive to their chemical environment. Here we explore the use of XPS as a fast technique to characterize EV membrane composition, with possible application in cancer research. Notably, we have focused on the nitrogen environment as an indicator of the relative abundance of pyridine-type bonding, primary, secondary and tertiary amines. Specifically, we have analyzed how tumoral and healthy cells have different nitrogen chemical environments that can indicate the presence or absence of malignancy. In addition, a collection of human serum samples from cancer patients and healthy donors was also analyzed. The differential XPS analysis of EVs collected from patients confirmed that the patterns of amine evolution could be related to markers of cancer disease, opening the possibility of their use as a non-invasive blood biomarker. info:eu-repo/grantAgreement/ES/DGA/B054-12 info:eu-repo/grantAgreement/ES/DGA-FEDER/Una manera de hacer Europa info:eu-repo/grantAgreement/ES/DGA-FSE/Building Europe from Aragon info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE info:eu-repo/grantAgreement/ES/ISCIII DTS21-00130 info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.5 2023 ONCOLOGY 78 / 322 = 0.242 2023 Q1 T1 1.391 2023 Oncology 2023 Q1 Cancer Research 2023 Q2 8.0 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Martín-Pardillos, Ana Universidad de Zaragoza Irusta, Silvia Universidad de Zaragoza (orcid)0000-0002-2966-9088 Sebastián, Víctor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Cebolla, Vicente Luis Pazo-Cid, Roberto Martín-Duque, Pilar Universidad de Zaragoza (orcid)0000-0003-4160-9720 Santamaría, Jesús Universidad de Zaragoza (orcid)0000-0002-8701-9745 1013 275 Universidad de Zaragoza Dpto. Cirugía Área Estomatología 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 15, 9 (2023), 2479 [18 pp.] Cancers Cancers 2072-6694 3728735 http://zaguan.unizar.es/record/126481/files/texto_completo.pdf Versión publicada 2731911 http://zaguan.unizar.es/record/126481/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:126481 articulos driver 2024-11-22-12:11:53 ARTICLE