126539 20241125101145.0 doi 10.3390/gels9060505 sideral 133976 ART-2023-133976 eng Pele, Karinna Georgiana Hydrocolloids of egg white and gelatin as a platform for hydrogel-based tissue engineering 2023 Access copy available to the general public Unrestricted Innovative materials are needed to produce scaffolds for various tissue engineering and regenerative medicine (TERM) applications, including tissue models. Materials derived from natural sources that offer low production costs, easy availability, and high bioactivity are highly preferred. Chicken egg white (EW) is an overlooked protein-based material. Whilst its combination with the biopolymer gelatin has been investigated in the food technology industry, mixed hydrocolloids of EW and gelatin have not been reported in TERM. This paper investigates these hydrocolloids as a suitable platform for hydrogel-based tissue engineering, including 2D coating films, miniaturized 3D hydrogels in microfluidic devices, and 3D hydrogel scaffolds. Rheological assessment of the hydrocolloid solutions suggested that temperature and EW concentration can be used to fine-tune the viscosity of the ensuing gels. Fabricated thin 2D hydrocolloid films presented globular nano-topography and in vitro cell work showed that the mixed hydrocolloids had increased cell growth compared with EW films. Results showed that hydrocolloids of EW and gelatin can be used for creating a 3D hydrogel environment for cell studies inside microfluidic devices. Finally, 3D hydrogel scaffolds were fabricated by sequential temperature-dependent gelation followed by chemical cross-linking of the polymeric network of the hydrogel for added mechanical strength and stability. These 3D hydrogel scaffolds displayed pores, lamellae, globular nano-topography, tunable mechanical properties, high affinity for water, and cell proliferation and penetration properties. In conclusion, the large range of properties and characteristics of these materials provide a strong potential for a large variety of TERM applications, including cancer models, organoid growth, compatibility with bioprinting, or implantable devices. info:eu-repo/grantAgreement/ES/DGA/E09-23R info:eu-repo/grantAgreement/ES/DGA/LMP176-21 info:eu-repo/grantAgreement/EC/H2020/101018587/EU/Individual and Collective Migration of the Immune Cellular System/ICoMICS This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101018587-ICoMICS info:eu-repo/grantAgreement/ES/MICINN/PID2020-113819RB-I00/AEI/10.13039/501100011033 info:eu-repo/grantAgreement/ES/MICINN/RYC2021-033490-I info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/PID2021-122409OB-C21 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.0 2023 POLYMER SCIENCE 14 / 95 = 0.147 2023 Q1 T1 0.674 2023 Polymers and Plastics 2023 Q1 Organic Chemistry 2023 Q2 Bioengineering 2023 Q2 Biomaterials 2023 Q2 4.7 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Amaveda, Hippolyte Universidad de Zaragoza (orcid)0000-0003-2212-447X Mora, Mario Universidad de Zaragoza (orcid)0000-0003-4747-7327 Marcuello, Carlos (orcid)0000-0003-3459-8605 Lostao, Anabel (orcid)0000-0001-7460-5916 Alamán-Díez, Pilar Universidad de Zaragoza (orcid)0000-0003-1958-4432 Pérez-Huertas, Salvador Pérez, María Ángeles Universidad de Zaragoza (orcid)0000-0002-2901-4188 García-Aznar, José Manuel Universidad de Zaragoza (orcid)0000-0002-9864-7683 García-Gareta, Elena Universidad de Zaragoza (orcid)0000-0001-7062-9099 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 5001 065 Universidad de Zaragoza Dpto. Ciencia Tecnol.Mater.Fl. Área Cienc.Mater. Ingen.Metal. 9, 6 (2023), 505 [22 pp.] Gels Gels 2310-2861 8954723 http://zaguan.unizar.es/record/126539/files/texto_completo.pdf Versión publicada 2637248 http://zaguan.unizar.es/record/126539/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:126539 articulos driver 2024-11-22-12:04:20 ARTICLE