126591 20241125101138.0 doi 10.3390/ijms24087459 sideral 133876 ART-2023-133876 eng Pariente, Ana Identification of 7-Ketocholesterol-Modulated Pathways and Sterculic Acid Protective Effect in Retinal Pigmented Epithelium Cells by Using Genome-Wide Transcriptomic Analysis 2023 Access copy available to the general public Unrestricted Age-related macular degeneration (AMD) is the leading cause of blindness in developed countries. AMD is characterized by the formation of lipidic deposits between the retinal pigment epithelium (RPE) and the choroid called drusen. 7-Ketocholesterol (7KCh), an oxidized-cholesterol derivative, is closely related to AMD as it is one of the main molecules accumulated in drusen. 7KCh induces inflammatory and cytotoxic responses in different cell types, and a better knowledge of the signaling pathways involved in its response would provide a new perspective on the molecular mechanisms that lead to the development of AMD. Furthermore, currently used therapies for AMD are not efficient enough. Sterculic acid (SA) attenuates the 7KCh response in RPE cells and is presented as an alternative to improve these therapies. By using genome-wide transcriptomic analysis in monkey RPE cells, we have provided new insight into 7KCh-induced signaling in RPE cells, as well as the protective capacity of SA. 7KCh modulates the expression of several genes associated with lipid metabolism, endoplasmic reticulum stress, inflammation and cell death and induces a complex response in RPE cells. The addition of SA successfully attenuates the deleterious effect of 7KCh and highlights its potential for the treatment of AMD. info:eu-repo/grantAgreement/ES/ISCIII/CPII20-00029 info:eu-repo/grantAgreement/ES/ISCIII-FEDER/PI19/01805 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.9 2023 BIOCHEMISTRY & MOLECULAR BIOLOGY 66 / 313 = 0.211 2023 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 68 / 231 = 0.294 2023 Q2 T1 1.179 2023 Medicine (miscellaneous) 2023 Q1 Physical and Theoretical Chemistry 2023 Q1 Computer Science Applications 2023 Q1 Inorganic Chemistry 2023 Q1 Spectroscopy 2023 Q1 Organic Chemistry 2023 Q1 Molecular Biology 2023 Q2 Catalysis 2023 Q2 8.1 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pérez-Sala, Álvaro Ochoa, Rodrigo Bobadilla, Miriam Villanueva-Martínez, Ángela Peláez, Rafael Larráyoz, Ignacio M. 24, 8 (2023), 7459 [22 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 4327189 http://zaguan.unizar.es/record/126591/files/texto_completo.pdf Versión publicada 2781713 http://zaguan.unizar.es/record/126591/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:126591 articulos driver 2024-11-22-12:01:45 ARTICLE