000126595 001__ 126595
000126595 005__ 20241125101140.0
000126595 0247_ $$2doi$$a10.1111/1462-2920.16434
000126595 0248_ $$2sideral$$a134085
000126595 037__ $$aART-2023-134085
000126595 041__ $$aeng
000126595 100__ $$0(orcid)0009-0005-3815-865X$$aOlivan-Muro, Irene$$uUniversidad de Zaragoza
000126595 245__ $$aUnbalancing Zur (FurB)-mediated homeostasis in Anabaena sp. PCC7120: Consequences on metal trafficking, heterocyst development and biofilm formation
000126595 260__ $$c2023
000126595 5060_ $$aAccess copy available to the general public$$fUnrestricted
000126595 5203_ $$aZinc is required for the activity of many enzymes and plays an essential role in gene regulation and redox homeostasis. In Anabaena (Nostoc) sp. PCC7120, the genes involved in zinc uptake and transport are controlled by the metalloregulator Zur (FurB). Comparative transcriptomics of a zur mutant (Δzur) with the parent strain unveiled unexpected links between zinc homeostasis and other metabolic pathways. A notable increase in the transcription of numerous desiccation tolerance-related genes, including genes involved in the synthesis of trehalose and the transference of saccharide moieties, among many others, was detected. Biofilm formation analysis under static conditions revealed a reduced capacity of Δzur filaments to form biofilms compared to the parent strain, and such capacity was enhanced when Zur was overexpressed. Furthermore, microscopy analysis revealed that zur expression is required for the correct formation of the envelope polysaccharide layer in the heterocyst, as Δzur cells showed reduced staining with alcian blue compared to Anabaena sp. PCC7120. We suggest that Zur is an important regulator of the enzymes involved in the synthesis and transport of the envelope polysaccharide layer, influencing heterocyst development and biofilm formation, both relevant processes for cell division and interaction with substrates in its ecological niche.
000126595 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E35-20R$$9info:eu-repo/grantAgreement/ES/MCIU/PID2019-104889GB-I00
000126595 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000126595 590__ $$a4.3$$b2023
000126595 592__ $$a1.342$$b2023
000126595 591__ $$aMICROBIOLOGY$$b45 / 161 = 0.28$$c2023$$dQ2$$eT1
000126595 593__ $$aMicrobiology$$c2023$$dQ1
000126595 593__ $$aEcology, Evolution, Behavior and Systematics$$c2023$$dQ1
000126595 594__ $$a9.9$$b2023
000126595 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000126595 700__ $$0(orcid)0000-0002-1960-2672$$aSarasa-Buisan, Cristina
000126595 700__ $$0(orcid)0000-0002-0474-255X$$aGuio, Jorge$$uUniversidad de Zaragoza
000126595 700__ $$0(orcid)0000-0002-8134-0693$$aArenas, Jesús$$uUniversidad de Zaragoza
000126595 700__ $$0(orcid)0000-0001-6435-3540$$aSevilla, Emma$$uUniversidad de Zaragoza
000126595 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, Maria F.$$uUniversidad de Zaragoza
000126595 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000126595 7102_ $$11002$$2412$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Fisiología Vegetal
000126595 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000126595 773__ $$g25, 11 (2023), 2142-2162$$pEnviron. microbiol.$$tEnvironmental Microbiology$$x1462-2912
000126595 8564_ $$s2187380$$uhttps://zaguan.unizar.es/record/126595/files/texto_completo.pdf$$yVersión publicada
000126595 8564_ $$s2667196$$uhttps://zaguan.unizar.es/record/126595/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000126595 909CO $$ooai:zaguan.unizar.es:126595$$particulos$$pdriver
000126595 951__ $$a2024-11-22-12:02:16
000126595 980__ $$aARTICLE