126595 20241125101140.0 doi 10.1111/1462-2920.16434 sideral 134085 ART-2023-134085 eng Olivan-Muro, Irene Universidad de Zaragoza (orcid)0009-0005-3815-865X Unbalancing Zur (FurB)-mediated homeostasis in Anabaena sp. PCC7120: Consequences on metal trafficking, heterocyst development and biofilm formation 2023 Access copy available to the general public Unrestricted Zinc is required for the activity of many enzymes and plays an essential role in gene regulation and redox homeostasis. In Anabaena (Nostoc) sp. PCC7120, the genes involved in zinc uptake and transport are controlled by the metalloregulator Zur (FurB). Comparative transcriptomics of a zur mutant (Δzur) with the parent strain unveiled unexpected links between zinc homeostasis and other metabolic pathways. A notable increase in the transcription of numerous desiccation tolerance-related genes, including genes involved in the synthesis of trehalose and the transference of saccharide moieties, among many others, was detected. Biofilm formation analysis under static conditions revealed a reduced capacity of Δzur filaments to form biofilms compared to the parent strain, and such capacity was enhanced when Zur was overexpressed. Furthermore, microscopy analysis revealed that zur expression is required for the correct formation of the envelope polysaccharide layer in the heterocyst, as Δzur cells showed reduced staining with alcian blue compared to Anabaena sp. PCC7120. We suggest that Zur is an important regulator of the enzymes involved in the synthesis and transport of the envelope polysaccharide layer, influencing heterocyst development and biofilm formation, both relevant processes for cell division and interaction with substrates in its ecological niche. info:eu-repo/grantAgreement/ES/DGA/E35-20R info:eu-repo/grantAgreement/ES/MCIU/PID2019-104889GB-I00 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.3 2023 MICROBIOLOGY 45 / 161 = 0.28 2023 Q2 T1 1.342 2023 Microbiology 2023 Q1 Ecology, Evolution, Behavior and Systematics 2023 Q1 9.9 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sarasa-Buisan, Cristina (orcid)0000-0002-1960-2672 Guio, Jorge Universidad de Zaragoza (orcid)0000-0002-0474-255X Arenas, Jesús Universidad de Zaragoza (orcid)0000-0002-8134-0693 Sevilla, Emma Universidad de Zaragoza (orcid)0000-0001-6435-3540 Fillat, Maria F. Universidad de Zaragoza (orcid)0000-0001-8644-4574 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1002 412 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Fisiología Vegetal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 25, 11 (2023), 2142-2162 Environ. microbiol. Environmental Microbiology 1462-2912 2187380 http://zaguan.unizar.es/record/126595/files/texto_completo.pdf Versión publicada 2667196 http://zaguan.unizar.es/record/126595/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:126595 articulos driver 2024-11-22-12:02:16 ARTICLE