000126612 001__ 126612
000126612 005__ 20241125101201.0
000126612 0247_ $$2doi$$a10.3390/life13051085
000126612 0248_ $$2sideral$$a134097
000126612 037__ $$aART-2023-134097
000126612 041__ $$aeng
000126612 100__ $$0(orcid)0000-0002-8467-0356$$aBuey, Berta$$uUniversidad de Zaragoza
000126612 245__ $$aGut microbiota-derived short-chain fatty acids: novel regulators of intestinal serotonin transporter
000126612 260__ $$c2023
000126612 5060_ $$aAccess copy available to the general public$$fUnrestricted
000126612 5203_ $$aSerotonin (5-HT) is a key neurotransmitter synthesized both in the gut and the central nervous system. It exerts its signaling through specific receptors (5-HTR), which regulate numerous behaviors and functions such as mood, cognitive function, platelet aggregation, gastrointestinal motility, and inflammation. Serotonin activity is determined mainly by the extracellular availability of 5-HT, which is controlled by the serotonin transporter (SERT). Recent studies indicate that, by activation of innate immunity receptors, gut microbiota can modulate serotonergic signaling by SERT modulation. As part of its function, gut microbiota metabolize nutrients from diet to produce different by-products, including short-chain fatty acids (SCFAs): propionate, acetate, and butyrate. However, it is not known whether these SCFAs regulate the serotonergic system. The objective of this study was to analyze the effect of SCFAs on the gastrointestinal serotonergic system using the Caco-2/TC7 cell line that expresses SERT and several receptors constitutively. Cells were treated with different SCFAs concentrations, and SERT function and expression were evaluated. In addition, the expression of 5-HT receptors 1A, 2A, 2B, 3A, 4, and 7 was also studied. Our results show that the microbiota-derived SCFAs regulate intestinal serotonergic system, both individually and in combination, modulating the function and expression of SERT and the 5-HT1A, 5-HT2B, and 5-HT7 receptors expression. Our data highlight the role of gut microbiota in the modulation of intestinal homeostasis and suggest microbiome modulation as a potential therapeutic treatment for intestinal pathologies and neuropsychiatric disorders involving serotonin.
000126612 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A20-20R$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2018-BIO-04
000126612 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000126612 590__ $$a3.2$$b2023
000126612 592__ $$a0.713$$b2023
000126612 591__ $$aBIOLOGY$$b26 / 109 = 0.239$$c2023$$dQ1$$eT1
000126612 593__ $$aPaleontology$$c2023$$dQ1
000126612 593__ $$aSpace and Planetary Science$$c2023$$dQ2
000126612 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2023$$dQ2
000126612 593__ $$aEcology, Evolution, Behavior and Systematics$$c2023$$dQ2
000126612 594__ $$a4.3$$b2023
000126612 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000126612 700__ $$aForcén, Ana
000126612 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, Laura$$uUniversidad de Zaragoza
000126612 700__ $$0(orcid)0000-0001-5573-6144$$aLayunta, Elena$$uUniversidad de Zaragoza
000126612 700__ $$0(orcid)0000-0003-4758-3998$$aMesonero, Jose Emilio$$uUniversidad de Zaragoza
000126612 700__ $$0(orcid)0000-0002-5797-3909$$aLatorre, Eva$$uUniversidad de Zaragoza
000126612 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000126612 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000126612 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000126612 773__ $$g13, 5 (2023), 1085 [11 pp.]$$pLife (Basel)$$tLife$$x2075-1729
000126612 8564_ $$s1117609$$uhttps://zaguan.unizar.es/record/126612/files/texto_completo.pdf$$yVersión publicada
000126612 8564_ $$s2667388$$uhttps://zaguan.unizar.es/record/126612/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000126612 909CO $$ooai:zaguan.unizar.es:126612$$particulos$$pdriver
000126612 951__ $$a2024-11-22-12:11:54
000126612 980__ $$aARTICLE