126890 20241125101156.0 doi 10.1016/j.foodcont.2023.109868 sideral 134259 ART-2023-134259 eng Civera, Alba Universidad de Zaragoza (orcid)0000-0001-7719-6388 Development and validation of sensitive and rapid immunoassays to detect minute amounts of hazelnut in processed food and working surfaces 2023 Access copy available to the general public Unrestricted Hazelnut (Corylus avellana L.) represents one of the most allergenic nuts and it can be found as a hidden allergen in processed food due to cross contamination. Therefore, sensitive and specific analytical techniques are in high demand to be used in allergen risk management plans at food industry. In this study, sandwich ELISA and Lateral Flow Immunoassay (LFIA) to detect hazelnut have been developed based on the determination of Cor a 9, one of the most abundant and allergenic proteins of hazelnut. Results showed that cross-reactivity was only found with walnut and Pecan nut, which was lower than 0.1%. When analyzing food spiked with a hazelnut extract or blended with hazelnut flour, ELISA and LFIA were able to detect 0.1 ppm and 0.5 ppm of hazelnut protein with a recovery from 82 to 110%. ELISA and LFIA could also detect 0.15 and 0.6 ppm of hazelnut protein in baked cookies incurred with ground hazelnut, respectively. Furthermore, LFIA could detect 1.25 μg of hazelnut protein in working surfaces of stainless steel and melamine. The sandwich ELISA was in-house validated, showing acceptable results of precision. Likewise, ELISA and LFIA showed to be robust tests. The combined use of both assays could improve the allergen risk management plans in food industry to monitor the presence of hazelnut traces in raw ingredients, processed food and working surfaces. info:eu-repo/grantAgreement/ES/DGA-FEDER/A02-20R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.6 2023 FOOD SCIENCE & TECHNOLOGY 24 / 173 = 0.139 2023 Q1 T1 1.146 2023 Food Science 2023 Q1 Biotechnology 2023 Q1 12.2 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Galan-Malo, Patricia Universidad de Zaragoza Mata, Luis Tobajas, Ana P. Universidad de Zaragoza (orcid)0000-0002-2646-5733 Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X Pérez, María D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 152 (2023), 109868 [9 pp.] Food control Food Control 0956-7135 1098972 http://zaguan.unizar.es/record/126890/files/texto_completo.pdf Versión publicada 2553879 http://zaguan.unizar.es/record/126890/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:126890 articulos driver 2024-11-22-12:09:33 ARTICLE