000126942 001__ 126942 000126942 005__ 20241125101140.0 000126942 0247_ $$2doi$$a10.1186/s12917-023-03682-5 000126942 0248_ $$2sideral$$a134433 000126942 037__ $$aART-2023-134433 000126942 041__ $$aeng 000126942 100__ $$0(orcid)0000-0002-7857-9732$$aLuño, Victoria$$uUniversidad de Zaragoza 000126942 245__ $$aEffect of season on the dynamics of cat sperm DNA fragmentation 000126942 260__ $$c2023 000126942 5060_ $$aAccess copy available to the general public$$fUnrestricted 000126942 5203_ $$aBackground: Feline species undergo reproductive seasonality; thus, sperm characteristics, such as DNA integrity, can be affected by the photoperiod. This study was conducted to determine the effect of seasonal changes on sperm quality and on the dynamics of sperm DNA fragmentation. Epididymal spermatozoa were collected from 36 tomcats subjected to bilateral orchiectomy during breeding (BS) and non-breeding (NBS) seasons. Sperm samples were obtained by cutting the cauda epididymis and assessed for sperm motility, concentration, acrosome integrity, plasma membrane integrity and sperm morphology. Sperm DNA fragmentation was evaluated by the sperm chromatin dispersion test after 0, 6, and 24 h of incubation at 37 °C. Results: The total sperm motility and plasma membrane integrity values were greater during the BS, while the percentages of abnormal sperm and head defects were lesser (p < 0.05). No significant differences in DNA fragmentation were found between seasons after sperm collection. DNA damage was greater after 24 h of incubation at 37 °C in both seasons, although the percentage of spermatozoa with fragmented DNA was significantly lesser in the BS than in the NBS at 24 h (p < 0.05).Conclusions: The study suggests seasonal changes in some of the quality parameters of cat sperm. DNA fragmentation dynamics were affected by the time of incubation and reproductive season; therefore, this technique might be used as an additional tool to test the potential fertility of semen samples used in feline-assisted reproduction. 000126942 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/A17-17R-RAySA$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/2014-2020 000126942 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000126942 590__ $$a2.3$$b2023 000126942 592__ $$a0.658$$b2023 000126942 591__ $$aVETERINARY SCIENCES$$b28 / 167 = 0.168$$c2023$$dQ1$$eT1 000126942 593__ $$aVeterinary (miscellaneous)$$c2023$$dQ1 000126942 593__ $$aMedicine (miscellaneous)$$c2023$$dQ2 000126942 594__ $$a4.8$$b2023 000126942 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000126942 700__ $$0(orcid)0000-0002-1659-3387$$aMartínez, Felisa$$uUniversidad de Zaragoza 000126942 700__ $$aMuñoz, Andrea 000126942 700__ $$0(orcid)0000-0002-0797-1841$$aGil, Lydia$$uUniversidad de Zaragoza 000126942 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000126942 773__ $$g19 (2023), 113 [7 pp]$$pBMC Vet. Res.$$tBMC VETERINARY RESEARCH$$x1746-6148 000126942 8564_ $$s1007591$$uhttps://zaguan.unizar.es/record/126942/files/texto_completo.pdf$$yVersión publicada 000126942 8564_ $$s2324071$$uhttps://zaguan.unizar.es/record/126942/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000126942 909CO $$ooai:zaguan.unizar.es:126942$$particulos$$pdriver 000126942 951__ $$a2024-11-22-12:02:28 000126942 980__ $$aARTICLE