126942 20241125101140.0 doi 10.1186/s12917-023-03682-5 sideral 134433 ART-2023-134433 eng Luño, Victoria Universidad de Zaragoza (orcid)0000-0002-7857-9732 Effect of season on the dynamics of cat sperm DNA fragmentation 2023 Access copy available to the general public Unrestricted Background: Feline species undergo reproductive seasonality; thus, sperm characteristics, such as DNA integrity, can be affected by the photoperiod. This study was conducted to determine the effect of seasonal changes on sperm quality and on the dynamics of sperm DNA fragmentation. Epididymal spermatozoa were collected from 36 tomcats subjected to bilateral orchiectomy during breeding (BS) and non-breeding (NBS) seasons. Sperm samples were obtained by cutting the cauda epididymis and assessed for sperm motility, concentration, acrosome integrity, plasma membrane integrity and sperm morphology. Sperm DNA fragmentation was evaluated by the sperm chromatin dispersion test after 0, 6, and 24 h of incubation at 37 °C. Results: The total sperm motility and plasma membrane integrity values were greater during the BS, while the percentages of abnormal sperm and head defects were lesser (p < 0.05). No significant differences in DNA fragmentation were found between seasons after sperm collection. DNA damage was greater after 24 h of incubation at 37 °C in both seasons, although the percentage of spermatozoa with fragmented DNA was significantly lesser in the BS than in the NBS at 24 h (p < 0.05).Conclusions: The study suggests seasonal changes in some of the quality parameters of cat sperm. DNA fragmentation dynamics were affected by the time of incubation and reproductive season; therefore, this technique might be used as an additional tool to test the potential fertility of semen samples used in feline-assisted reproduction. info:eu-repo/grantAgreement/ES/DGA-FEDER/A17-17R-RAySA info:eu-repo/grantAgreement/ES/DGA-FEDER/2014-2020 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.3 2023 VETERINARY SCIENCES 28 / 167 = 0.168 2023 Q1 T1 0.658 2023 Veterinary (miscellaneous) 2023 Q1 Medicine (miscellaneous) 2023 Q2 4.8 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Martínez, Felisa Universidad de Zaragoza (orcid)0000-0002-1659-3387 Muñoz, Andrea Gil, Lydia Universidad de Zaragoza (orcid)0000-0002-0797-1841 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 19 (2023), 113 [7 pp] BMC Vet. Res. BMC VETERINARY RESEARCH 1746-6148 1007591 http://zaguan.unizar.es/record/126942/files/texto_completo.pdf Versión publicada 2324071 http://zaguan.unizar.es/record/126942/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:126942 articulos driver 2024-11-22-12:02:28 ARTICLE