127027 20241125101156.0 doi 10.1111/nmo.14639 sideral 134329 ART-2023-134329 eng Castro, Marta Universidad de Zaragoza (orcid)0000-0001-8584-3979 Radiographic and histopathological study of gastrointestinal dysmotility in lipopolysaccharide-induced sepsis in the rat 2023 Access copy available to the general public Unrestricted AbstractSepsis is a highly incident condition in which a cascade of proinflammatory cytokines is involved. One of its most frequent consequences is ileus, which can increase mortality. Animal models such as that induced by systemic administration of lipopolysaccharide (LPS) are useful to deeply evaluate this condition. The effects of sepsis on the gastrointestinal (GI) tract have been explored but, to our knowledge, in vivo studies showing the motor and histopathological consequences of endotoxemia in an integrated way are lacking. Our aim was to study in rats the effects of sepsis on GI motility, using radiographic methods, and to assess histological damage in several organs.MethodsMale rats were intraperitoneally injected with saline or E. coli LPS at 0.1, 1, or 5 mg kg−1. Barium sulfate was intragastrically administered, and X‐rays were performed 0–24 h afterwards. Several organs were collected for organography, histopathology, and immunohistochemistry studies.Key ResultsAll LPS doses caused gastroparesia, whereas changes in intestinal motility were dose‐and time‐dependent, with an initial phase of hypermotility followed by paralytic ileus. Lung, liver, stomach, ileum, and colon (but not spleen or kidneys) were damaged, and density of neutrophils and activated M2 macrophages and expression of cyclooxygenase 2 were increased in the colon 24 h after LPS 5 mg kg−1.Conclusions and InferencesUsing radiographic, noninvasive methods for the first time, we show that systemic LPS causes dose‐, time‐, and organ‐dependent GI motor effects. Sepsis‐induced GI dysmotility is a complex condition whose management needs to take its time‐dependent changes into account. info:eu-repo/grantAgreement/ES/DGA/B04-17R info:eu-repo/grantAgreement/ES/MICINN/SAF2012-40075-C02-01 info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987-R info:eu-repo/grantAgreement/ES/MINECO/SAF2017-83120-C2-1-R info:eu-repo/grantAgreement/ES/UZ/JIUZ-2015-BIO-02 info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 3.5 2023 CLINICAL NEUROLOGY 70 / 280 = 0.25 2023 Q1 T1 NEUROSCIENCES 110 / 310 = 0.355 2023 Q2 T2 GASTROENTEROLOGY & HEPATOLOGY 43 / 143 = 0.301 2023 Q2 T1 1.312 2023 Endocrine and Autonomic Systems 2023 Q1 Physiology 2023 Q1 Gastroenterology 2023 Q1 7.8 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Valero, Marta Sofía Universidad de Zaragoza (orcid)0000-0002-2231-7565 López-Tofiño, Yolanda López-Gómez, Laura Girón, Rocío Martín-Fontelles, María Isabel Uranga, José A. Abalo, Raquel 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 35, 10 (2023), e14639 [15 pp] Neurogastroenterol. motil. NEUROGASTROENTEROLOGY AND MOTILITY 1350-1925 4378878 http://zaguan.unizar.es/record/127027/files/texto_completo.pdf Versión publicada 2366268 http://zaguan.unizar.es/record/127027/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:127027 articulos driver 2024-11-22-12:09:36 ARTICLE