000127040 001__ 127040
000127040 005__ 20240705134220.0
000127040 0247_ $$2doi$$a10.3389/fcell.2023.1155634
000127040 0248_ $$2sideral$$a134402
000127040 037__ $$aART-2023-134402
000127040 041__ $$aeng
000127040 100__ $$aDucreux, Bastien
000127040 245__ $$aAssessing the influence of distinct culture media on human pre-implantation development using single-embryo transcriptomics
000127040 260__ $$c2023
000127040 5060_ $$aAccess copy available to the general public$$fUnrestricted
000127040 5203_ $$aThe use of assisted reproductive technologies is consistently rising across the world. However, making an informed choice on which embryo culture medium should be preferred to ensure satisfactory pregnancy rates and the health of future children critically lacks scientific background. In particular, embryos within their first days of development are highly sensitive to their micro-environment, and it is unknown how their transcriptome adapts to different embryo culture compositions. Here, we determined the impact of culture media composition on gene expression in human pre-implantation embryos. By employing single-embryo RNA-sequencing after 2 or 5 days of the post-fertilization culture in different commercially available media (Ferticult, Global, and SSM), we revealed medium-specific differences in gene expression changes. Embryos cultured pre-compaction until day 2 in Ferticult or Global media notably displayed 266 differentially expressed genes, which were related to essential developmental pathways. Herein, 19 of them could have a key role in early development, based on their previously described dynamic expression changes across development. When embryos were cultured after day 2 in the same media considered more suitable because of its amino acid enrichment, 18 differentially expressed genes thought to be involved in the transition from early to later embryonic stages were identified. Overall, the differences were reduced at the blastocyst stage, highlighting the ability of embryos conceived in a suboptimal in vitro culture medium to mitigate the transcriptomic profile acquired under different pre-compaction environments.
000127040 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000127040 592__ $$a1.576$$b2023
000127040 593__ $$aDevelopmental Biology$$c2023$$dQ1
000127040 593__ $$aCell Biology$$c2023$$dQ1
000127040 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000127040 700__ $$aBarberet, Julie
000127040 700__ $$aGuilleman, Magali
000127040 700__ $$0(orcid)0000-0001-8655-9267$$aPérez-Palacios, Raquel$$uUniversidad de Zaragoza
000127040 700__ $$aTeissandier, Aurélie
000127040 700__ $$aBourc’his, Déborah
000127040 700__ $$aFauque, Patricia
000127040 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000127040 773__ $$g11 (2023), [14 pp.]$$tFrontiers in Cell and Developmental Biology$$x2296-634X
000127040 8564_ $$s3350289$$uhttps://zaguan.unizar.es/record/127040/files/texto_completo.pdf$$yVersión publicada
000127040 8564_ $$s2125861$$uhttps://zaguan.unizar.es/record/127040/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000127040 909CO $$ooai:zaguan.unizar.es:127040$$particulos$$pdriver
000127040 951__ $$a2024-07-05-12:53:57
000127040 980__ $$aARTICLE