000127112 001__ 127112 000127112 005__ 20241125101155.0 000127112 0247_ $$2doi$$a10.1371/journal.pone.0289761 000127112 0248_ $$2sideral$$a134504 000127112 037__ $$aART-2023-134504 000127112 041__ $$aeng 000127112 100__ $$0(orcid)0000-0002-1960-2672$$aSarasa-Buisan, Cristina 000127112 245__ $$aExpanding the FurC (PerR) regulon in Anabaena (Nostoc) sp. PCC 7120: Genome-wide identification of novel direct targets uncovers FurC participation in central carbon metabolism regulation 000127112 260__ $$c2023 000127112 5060_ $$aAccess copy available to the general public$$fUnrestricted 000127112 5203_ $$aFurC (PerR, Peroxide Response Regulator) from Anabaena sp. PCC 7120 (also known as Nostoc sp. PCC 7120) is a master regulator engaged in the modulation of relevant processes including the response to oxidative stress, photosynthesis and nitrogen fixation. Previous differential gene expression analysis of a furC-overexpressing strain (EB2770FurC) allowed the inference of a putative FurC DNA-binding consensus sequence. In the present work, more data concerning the regulon of the FurC protein were obtained through the searching of the putative FurC-box in the whole Anabaena sp. PCC 7120 genome. The total amount of novel FurC-DNA binding sites found in the promoter regions of genes with known function was validated by electrophoretic mobility shift assays (EMSA) identifying 22 new FurC targets. Some of these identified targets display relevant roles in nitrogen fixation (hetR and hgdC) and carbon assimilation processes (cmpR, glgP1 and opcA), suggesting that FurC could be an additional player for the harmonization of carbon and nitrogen metabolisms. Moreover, differential gene expression of a selection of newly identified FurC targets was measured by Real Time RT-PCR in the furC-overexpressing strain (EB2770FurC) comparing to Anabaena sp. PCC 7120 revealing that in most of these cases FurC could act as a transcriptional activator. 000127112 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E35-20R$$9info:eu-repo/grantAgreement/ES/MCIU/PID2019-104889GB-I00 000127112 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000127112 590__ $$a2.9$$b2023 000127112 592__ $$a0.839$$b2023 000127112 591__ $$aMULTIDISCIPLINARY SCIENCES$$b32 / 134 = 0.239$$c2023$$dQ1$$eT1 000127112 593__ $$aMultidisciplinary$$c2023$$dQ1 000127112 594__ $$a6.2$$b2023 000127112 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000127112 700__ $$0(orcid)0000-0002-0474-255X$$aGuío, Jorge$$uUniversidad de Zaragoza 000127112 700__ $$0(orcid)0000-0002-2742-3711$$aPeleato, M. Luisa 000127112 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, María F.$$uUniversidad de Zaragoza 000127112 700__ $$0(orcid)0000-0001-6435-3540$$aSevilla, Emma$$uUniversidad de Zaragoza 000127112 7102_ $$11002$$2412$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Fisiología Vegetal 000127112 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000127112 773__ $$g18, 8 (2023), e0289761 [19 pp]$$pPLoS One$$tPLoS ONE$$x1932-6203 000127112 8564_ $$s2049496$$uhttps://zaguan.unizar.es/record/127112/files/texto_completo.pdf$$yVersión publicada 000127112 8564_ $$s2215948$$uhttps://zaguan.unizar.es/record/127112/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000127112 909CO $$ooai:zaguan.unizar.es:127112$$particulos$$pdriver 000127112 951__ $$a2024-11-22-12:08:40 000127112 980__ $$aARTICLE