doi:10.1021/acs.analchem.2c05389engSu, Q.Vera, P.Nerín, C.Combination of structure databases, In silico fragmentation, and MS/MS libraries for untargeted screening of non-volatile migrants from recycled high-density polyethylene milk bottlesART-2023-134503Chemical contamination is one of the major obstacles for mechanical recycling of plastics. In this article, we built and open-sourced an in-house MS/MS library containing more than 500 plastic-related chemicals and developed mspcompiler, an R package, for the compilation of various libraries. We then proposed a workflow to process untargeted screening data acquired by liquid chromatography high-resolution mass spectrometry. These tools were subsequently employed to data originating from recycled high-density polyethylene (rHDPE) obtained from milk bottles. A total of 83 compounds were identified, with 66 easily annotated by making use of our in-house MS/MS libraries and the mspcompiler R package. In silico fragmentation combined with data obtained from gas chromatography–mass spectrometry and lists of chemicals related to plastics were used to identify those remaining unknown. A pseudo-multiple reaction monitoring method was also applied to sensitively target and screen the identified chemicals in the samples. Quantification results demonstrated that a good sorting of postconsumer materials and a better recycling technology may be necessary for food contact applications. Removal or reduction of non-volatile substances, such as octocrylene and 2-ethylhexyl-4-methoxycinnamate, is still challenging but vital for the safe use of rHDPE as food contact materials.2023http://zaguan.unizar.es/record/12711310.1021/acs.analchem.2c05389http://zaguan.unizar.es/record/127113oai:zaguan.unizar.es:127113info:eu-repo/grantAgreement/ES/DGA-FSE/T53-20Rinfo:eu-repo/grantAgreement/ES/MINECO/RTI2018-097805-B-I00ANALYTICAL CHEMISTRY 95, 23 (2023), 8780-8788byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess