000127157 001__ 127157
000127157 005__ 20230907110833.0
000127157 037__ $$aTAZ-TFM-2023-389
000127157 041__ $$aspa
000127157 1001_ $$aPaúl Bernal, Jorge
000127157 24200 $$aDevelopment of bispecific NK cell engagers (BiKE) against Tn antigen for cancer immunotherapy
000127157 24500 $$aDesarrollo de anticuerpos bi-específicos de unión a células NK (BiKE) frente al antígeno Tn para inmunoterapia en cáncer
000127157 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2023
000127157 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/
000127157 520__ $$aIntroduction and objective. BiKEs (bispecific killer cell engagers) are a new form of immunotherapy that consists of two connected scFv. One scFv recognizes a tumor antigen and the other binds to CD16 on the NK cell membrane, thus NK cells are activated and specifically target the tumor. It’s thought that BiKEs would have the advantage over monoclonal antibodies of more easily penetrating solid tumors and generating a stronger stimulus to NK cells that would be capable of activating them even in the immunosuppressive conditions of the tumor microenvironment. The objective of this work is to design and recombinantly express two BiKEs, in addition to three other proteins, using the Tn antigen as a target. This antigen is an aberrant glycosylation pattern specific to tumor cells and highly abundant in tumors of epithelial origin.<br />Results. The 5 proteins have been expressed in HEK 293 6F cells with a good yield and degree of purity. We have been able to demonstrate that these proteins are correctly folded, dimerized and glycosylated. We also have probed that the scFvs of the BiKEs recognize Tn antigen as is described in the literature. However, the ability of BiKEs to activate NK cells against tumor cells in in vitro cultures couldn’t be demonstrated due to the lack of an adequate positive control.<br /><br />
000127157 521__ $$aMáster en Biología Molecular y Celular
000127157 540__ $$aDerechos regulados por licencia Creative Commons
000127157 700__ $$aPardo Jimeno, Julián$$edir.
000127157 700__ $$aHurtado Guerrero, Ramón$$edir.
000127157 7102_ $$aUniversidad de Zaragoza$$b $$c
000127157 8560_ $$f778952@unizar.es
000127157 8564_ $$s7389301$$uhttps://zaguan.unizar.es/record/127157/files/TAZ-TFM-2023-389.pdf$$yMemoria (spa)
000127157 909CO $$ooai:zaguan.unizar.es:127157$$pdriver$$ptrabajos-fin-master
000127157 950__ $$a
000127157 951__ $$adeposita:2023-09-07
000127157 980__ $$aTAZ$$bTFM$$cCIEN
000127157 999__ $$a20230612233352.CREATION_DATE