000127650 001__ 127650
000127650 005__ 20241125101143.0
000127650 0247_ $$2doi$$a10.14670/HH-18-656
000127650 0248_ $$2sideral$$a134746
000127650 037__ $$aART-2023-134746
000127650 041__ $$aeng
000127650 100__ $$aDíaz de Cerio, Maria
000127650 245__ $$aCold-shock proteins accumulate in centrosomes and their expression and primary cilium morphology are regulated by hypothermia and shear stress
000127650 260__ $$c2023
000127650 5060_ $$aAccess copy available to the general public$$fUnrestricted
000127650 5203_ $$aPrimary cilia act as cellular sensors for multiple extracellular stimuli and regulate many intracellular signaling pathways in response. Here we investigate whether the cold-shock proteins (CSPs), CIRP and RBM3, are present in the primary cilia and the physiological consequences of such a relationship. R28, an immortalized retinal precursor cell line, was stained with antibodies against CIRP, RBM3, and ciliary markers. Both CSPs were found in intimate contact with the basal body of the cilium during all stages of the cell cycle, including migrating with the centrosome during mitosis. In addition, the morphological and physiological manifestations of exposing the cells to hypothermia and shear stress were investigated. Exposure to moderately cold (32°C) temperatures, the hypothermia mimetic small molecule zr17-2, or to shear stress resulted in a significant reduction in the number and length of primary cilia. In addition, shear stress induced expression of CIRP and RBM3 in a complex pattern depending on the specific protein, flow intensity, and type of flow (laminar versus oscillatory). Flow-mediated CSP overexpression was detected by qRT-PCR and confirmed by Western blot, at least for CIRP. Furthermore, analysis of public RNA Seq databases on flow experiments confirmed an increase of CIRP and RBM3 expression following exposure to shear stress in renal cell lines. In conclusion, we found that CSPs are integral components of the centrosome and that they participate in cold and shear stress sensing. Histol Histopathol
000127650 536__ $$9info:eu-repo/grantAgreement/EC/H2020/876190/EU/Accelerating Innovation in Microfabricated Medical Devices/Moore4Medical$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 876190-Moore4Medical
000127650 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000127650 590__ $$a2.5$$b2023
000127650 592__ $$a0.571$$b2023
000127650 591__ $$aPATHOLOGY$$b37 / 88 = 0.42$$c2023$$dQ2$$eT2
000127650 593__ $$aPathology and Forensic Medicine$$c2023$$dQ2
000127650 591__ $$aCELL BIOLOGY$$b148 / 205 = 0.722$$c2023$$dQ3$$eT3
000127650 593__ $$aHistology$$c2023$$dQ2
000127650 594__ $$a3.9$$b2023
000127650 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000127650 700__ $$0(orcid)0000-0003-0156-4230$$aOliván García, Sara$$uUniversidad de Zaragoza
000127650 700__ $$aOchoa Garrido, Ignacio
000127650 700__ $$aGracia-San Martin, Josune
000127650 700__ $$aMartinez, Alfredo
000127650 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000127650 773__ $$g39 (2023), 447-462$$pHistol. histopathol.$$tHistology and histopathology$$x0213-3911
000127650 8564_ $$s1029469$$uhttps://zaguan.unizar.es/record/127650/files/texto_completo.pdf$$yPostprint
000127650 8564_ $$s919868$$uhttps://zaguan.unizar.es/record/127650/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000127650 909CO $$ooai:zaguan.unizar.es:127650$$particulos$$pdriver
000127650 951__ $$a2024-11-22-12:03:18
000127650 980__ $$aARTICLE