127684 20241125101157.0 doi 10.3390/ani13162565 sideral 134770 ART-2023-134770 eng Contreras-Solís, Ignacio The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes 2023 Access copy available to the general public Unrestricted This study analyzed the effects of dietary supplementation with by-pass linseed oil (LO; rich in α-linolenic acid) on maternal antioxidant systems at Days 14 and 16 of pregnancy in Sarda ewes. This trial used sixteen dry ewes. Eight ewes (CT group) were fed with a control diet without LO, and eight ewes (LO group) were fed with a diet supplemented with LO (10.8 g of α-linolenic acid/ewe/day). Both diets had similar crude protein and energy levels. The experiment included 10 days of an adaptation period and 31 days of a supplementation period. This supplementation period was divided into Period −2 (from Day −15 to −8), Period −1 (from Day −7 to −1; before synchronized mating period/Day 0), Period +1 (from Day +1 to + 7 after mating), and Period +2 (from Day +8 to +15 after mating). Estrous synchronization was induced in all the ewes using an intravaginal sponge (45 mg fluorgestone acetate) for 14 days and equine chorionic gonadotropin (350 UI/ewe) at the end of the treatment. On Days 14 (CT, N = 4; LO, N = 4) and 16 (CT, N = 4; LO, N = 4) after mating, the ewes were slaughtered. Samples of plasma, uterine, and luteal tissues were collected. Thiols, total antioxidant activity (TEAC), superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were measured. On Day 16, thiol and TEAC in luteal tissues were higher in the LO group when compared with the control one (p < 0.05). Moreover, TEAC was higher for the LO group in uterine tissues on Days 14 and 16 (p < 0.05). SOD activity was higher in the LO group in luteal and uterine tissues on Day 14 and Day 16, respectively (p < 0.001). On Day 16, uterine MDA content was lower for the LO group (p < 0.001). No differences were found between groups at the plasmatic level. However, the by-pass LO supplementation enhanced the analyzed antioxidant parameters in luteal and uterine tissues. In conclusion, these results demonstrate that by-pass LO supplementation exerted a positive effect on antioxidative defenses on maternal structures during the embryo-maternal recognition period in ewes. Thus, this could contribute to improving the maternal environment during the embryo-maternal recognition period in mammals. info:eu-repo/grantAgreement/EC/H2020/832326/EU/Effect of polyinsatured fatty acids omega-3 (PUFAS n-3) supplementation during early embryo development on embryo viability in (mammals) sheep./NUTREPHEALTH This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 832326-NUTREPHEALTH info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.7 2023 VETERINARY SCIENCES 16 / 167 = 0.096 2023 Q1 T1 AGRICULTURE, DAIRY & ANIMAL SCIENCE 10 / 80 = 0.125 2023 Q1 T1 0.698 2023 Veterinary (miscellaneous) 2023 Q1 Animal Science and Zoology 2023 Q1 4.9 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pasciu, Valeria Porcu, Cristian Sotgiu, Francesca D. Todorova, Neda Baralla, Elena Mara, Laura Gallus, Marilia Cabiddu, Andrea Dattena, Maria Abecia, José Alfonso Universidad de Zaragoza (orcid)0000-0003-2827-3054 Berlinguer, Fiammetta 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 13, 16 (2023), 2565 [14 pp.] Animals (Basel) Animals 2076-2615 1643584 http://zaguan.unizar.es/record/127684/files/texto_completo.pdf Versión publicada 2771050 http://zaguan.unizar.es/record/127684/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:127684 articulos driver 2024-11-22-12:09:45 ARTICLE