127704 20251107103406.0 doi 10.1016/j.seppur.2023.124496 sideral 134756 ART-2023-134756 eng Artigas-Hernández, Diego Using eutectic solvents for extracting astaxanthin from dry biomass of Xanthophyllomyces dendrorhous pretreated by pulsed electric fields 2023 Access copy available to the general public Unrestricted The healthy properties of astaxanthin (AST) together with the growing interest in products of natural origin have motivated the development of sustainable extraction procedures from biomass. For this, it is essential to establish mechanisms that allow overcoming the barrier of the cell envelopes as well as the substitution of the conventional solvents. This work is focused on the evaluation of pulse electric fields (PEFs) as a fresh biomass pretreatment to enhance the efficacy of eutectic mixtures (ESs) as eco-friendly solvents for extracting AST from the freeze-dried yeast Xanthophyllomyces dendrorhous. The results showed a positive effect on the extraction efficiency of the pulse treatment and subsequent incubation stage. The thymol/salol system was the best solvent and the most favorable composition, temperature and extraction time were calculated. The efficiency reached 79% in the PEF-treated cells after incubation. The antioxidant capacity of AST in the eutectic mixture was determined and an IC50 value of 0.02 μg/mL was obtained. A synergistic effect between AST and thymol was observed. The main conclusion of the work is that consecutively using PEFs as an electroporation technique and ESs as solvents allows us to obtain a high extraction efficiency of AST from dry yeast. info:eu-repo/grantAgreement/ES/DGA/E31-23R info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 8.2 2023 ENGINEERING, CHEMICAL 15 / 170 = 0.088 2023 Q1 T1 1.533 2023 Filtration and Separation 2023 Q1 Analytical Chemistry 2023 Q1 14.0 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Berzosa, Alejandro Universidad de Zaragoza Aguilar-Machado, Diederich Raso, Javier Universidad de Zaragoza (orcid)0000-0003-3957-9091 Artal, Manuela Universidad de Zaragoza (orcid)0000-0003-1810-9488 2012 755 Universidad de Zaragoza Dpto. Química Física Área Química Física 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 324 (2023), 124496 [11 pp.] Sep. Purif. Technol. Separation and Purification Technology 1383-5866 4415263 http://zaguan.unizar.es/record/127704/files/texto_completo.pdf Versión publicada 2647675 http://zaguan.unizar.es/record/127704/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:127704 articulos driver 2025-11-07-10:32:11 ARTICLE