000127760 001__ 127760
000127760 005__ 20241125101132.0
000127760 0247_ $$2doi$$a10.3390/ijms241612552
000127760 0248_ $$2sideral$$a134909
000127760 037__ $$aART-2023-134909
000127760 041__ $$aeng
000127760 100__ $$aAbuobeid, Roubi
000127760 245__ $$aDifferentially Expressed Genes in Response to a Squalene-Supplemented Diet Are Accurate Discriminants of Porcine Non-Alcoholic Steatohepatitis
000127760 260__ $$c2023
000127760 5060_ $$aAccess copy available to the general public$$fUnrestricted
000127760 5203_ $$aSqualene is the major unsaponifiable component of virgin olive oil, the fat source of the Mediterranean diet. To evaluate its effect on the hepatic transcriptome, RNA sequencing was carried out in two groups of male Large White x Landrace pigs developing nonalcoholic steatohepatitis by feeding them a high fat/cholesterol/fructose and methionine and choline-deficient steatotic diet or the same diet with 0.5% squalene. Hepatic lipids, squalene content, steatosis, activity (ballooning + inflammation), and SAF (steatosis + activity + fibrosis) scores were analyzed. Pigs receiving the latter diet showed hepatic squalene accumulation and twelve significantly differentially expressed hepatic genes (log2 fold change < 1.5 or <1.5) correlating in a gene network. These pigs also had lower hepatic triglycerides and lipid droplet areas and higher cellular ballooning. Glutamyl aminopeptidase (ENPEP) was correlated with triglyceride content, while alpha-fetoprotein (AFP), neutralized E3 ubiquitin protein ligase 3 (NEURL3), 2′-5′-oligoadenylate synthase-like protein (OASL), and protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B) were correlated with activity reflecting inflammation and ballooning, and NEURL3 with the SAF score. AFP, ENPEP, and PPP1R1B exhibited a remarkably strong discriminant power compared to those pathological parameters in both experimental groups. Moreover, the expression of PPP1R1B, TMEM45B, AFP, and ENPEP followed the same pattern in vitro using human hepatoma (HEPG2) and mouse liver 12 (AML12) cell lines incubated with squalene, indicating a direct effect of squalene on these expressions. These findings suggest that squalene accumulated in the liver is able to modulate gene expression changes that may influence the progression of non-alcoholic steatohepatitis.
000127760 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B16-23R$$9info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/MICINN AEI/PID2019-104915RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN AEI/PID2022-104915RB-I00
000127760 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000127760 590__ $$a4.9$$b2023
000127760 592__ $$a1.179$$b2023
000127760 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 313 = 0.211$$c2023$$dQ1$$eT1
000127760 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1
000127760 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b68 / 231 = 0.294$$c2023$$dQ2$$eT1
000127760 593__ $$aPhysical and Theoretical Chemistry$$c2023$$dQ1
000127760 593__ $$aComputer Science Applications$$c2023$$dQ1
000127760 593__ $$aInorganic Chemistry$$c2023$$dQ1
000127760 593__ $$aSpectroscopy$$c2023$$dQ1
000127760 593__ $$aOrganic Chemistry$$c2023$$dQ1
000127760 593__ $$aMolecular Biology$$c2023$$dQ2
000127760 593__ $$aCatalysis$$c2023$$dQ2
000127760 594__ $$a8.1$$b2023
000127760 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000127760 700__ $$0(orcid)0000-0002-4665-9674$$aHerrera-Marcos, Luis V.$$uUniversidad de Zaragoza
000127760 700__ $$aArnal, Carmen
000127760 700__ $$aBidooki, Seyed Hesamoddin$$uUniversidad de Zaragoza
000127760 700__ $$0(orcid)0000-0002-9023-741X$$aSánchez-Marco, Javier
000127760 700__ $$aLasheras, Roberto
000127760 700__ $$0(orcid)0000-0002-5841-0462$$aSurra, Joaquín C.$$uUniversidad de Zaragoza
000127760 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, María Jesús$$uUniversidad de Zaragoza
000127760 700__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, Roberto$$uUniversidad de Zaragoza
000127760 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, Jesús$$uUniversidad de Zaragoza
000127760 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000127760 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000127760 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000127760 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000127760 773__ $$g24, 16 (2023), 12552 [20 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000127760 8564_ $$s3550426$$uhttps://zaguan.unizar.es/record/127760/files/texto_completo.pdf$$yVersión publicada
000127760 8564_ $$s2843542$$uhttps://zaguan.unizar.es/record/127760/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000127760 909CO $$ooai:zaguan.unizar.es:127760$$particulos$$pdriver
000127760 951__ $$a2024-11-22-11:59:23
000127760 980__ $$aARTICLE