127895 20241125101129.0 doi 10.1039/d3na00229b sideral 135124 ART-2023-135124 eng Gatti, Lodovico Pivotal role of the protein corona in the cell uptake of fluorinated nanoparticles with increased sensitivity for19F-MR imaging 2023 Access copy available to the general public Unrestricted In vivo cell tracking by non-invasive imaging technologies is needed to accelerate the clinical translation of innovative cell-based therapies. In this regard, 19F-MRI has recently gained increased attention for unbiased localization of labeled cells over time. To push forward the use of 19F-MRI for cell tracking, the development of highly performant 19F-probes is required. PLGA-based NPs containing PERFECTA, a multibranched superfluorinated molecule with an optimal MRI profile thanks to its 36 magnetically equivalent fluorine atoms, are promising 19F-MRI probes. In this work we demonstrate the importance of the surface functionalization of these NPs in relation to their interaction with the biological environment, stressing the pivotal role of the formation of the protein corona (PC) in their cellular labelling efficacy. In particular, our studies showed that the formation of PC NPs strongly promotes the cellular internalization of these NPs in microglia cells. We advocate that the formation of PC NPs in the culture medium can be a key element to be used for the optimization of cell labelling with a considerable increase of the detection sensitivity by 19F-MRI. info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 4.6 2023 MATERIALS SCIENCE, MULTIDISCIPLINARY 141 / 439 = 0.321 2023 Q2 T1 NANOSCIENCE & NANOTECHNOLOGY 60 / 141 = 0.426 2023 Q2 T2 CHEMISTRY, MULTIDISCIPLINARY 69 / 231 = 0.299 2023 Q2 T1 0.953 2023 Atomic and Molecular Physics, and Optics 2023 Q1 Engineering (miscellaneous) 2023 Q1 Materials Science (miscellaneous) 2023 Q1 Chemistry (miscellaneous) 2023 Q1 Bioengineering 2023 Q2 8.0 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Chirizzi, Cristina Rotta, Giulia Milesi, Pietro Sancho-Albero, María Universidad de Zaragoza (orcid)0000-0001-8762-5457 Sebastián, Victor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Mondino, Anna Santamaría, Jesús Universidad de Zaragoza (orcid)0000-0002-8701-9745 Metrangolo, Pierangelo Chaabane, Linda Baldelli Bombelli, Francesca 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5, 14 (2023), 3749-3760 Nanoscale Advances 2516-0230 1029197 http://zaguan.unizar.es/record/127895/files/texto_completo.pdf Versión publicada 2894760 http://zaguan.unizar.es/record/127895/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:127895 articulos driver 2024-11-22-11:58:25 ARTICLE