000127921 001__ 127921
000127921 005__ 20241125101144.0
000127921 0247_ $$2doi$$a10.3390/biomedicines11082173
000127921 0248_ $$2sideral$$a135081
000127921 037__ $$aART-2023-135081
000127921 041__ $$aeng
000127921 100__ $$aAmrane-Abider, Meriem
000127921 245__ $$aBioactive compounds and in vitro antioxidant and anticoccidial activities of Opuntia ficus-indica flower extracts
000127921 260__ $$c2023
000127921 5060_ $$aAccess copy available to the general public$$fUnrestricted
000127921 5203_ $$aThe objective of the present study is to identify the biochemical compounds extracted from OFI flowers using ultra-high-performance liquid chromatography–electrospray ionization quadrupole time-of-flight mass spectrometry and to evaluate their in vitro antioxidant activities and anticoccidial effects on the destruction of Eimeria oocysts isolated from naturally infected chickens. A domestic microwave was used with a refrigerant to condense the vapors generated during the extraction. The flavonoid and phenolic compound contents of the OFI flowers were determined according to standard methods. DPPH radical and H2O2 scavenging capacities were used to assess the antioxidant activity. Regarding the anticoccidial activity, the Eimeria spp. oocysts used were isolated from the fresh feces of infected broilers and were determined in triplicate by incubation at an ambient temperature for 24 h. The results highlighted the considerable influence of the optimized acetone concentration, ratio, irradiation time, and microwave power parameters on the phenolic content and antioxidant activities. Our results revealed significant matches between the predicted and experimental values of the models. Molecular analysis revealed the presence of several biophenol classes such as quercetin, isorhamnetin 3-O-rutinoside, and quercetin-3-O-rutinoside. OFI flower extracts inhibited sporulation and damaged the morphology of Eimeria oocysts compared with normal sporulated Eimeria oocysts containing sporocysts. In conclusion, the optimized conditions were validated and found to fit very well with the experimental values. These findings suggest that the flowers of OFI should be considered sources of antioxidants. The results of the present study revealed that OFI flower extracts have anticoccidial activities against Eimeria-spp.-induced infection in broiler chickens.
000127921 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000127921 590__ $$a3.9$$b2023
000127921 592__ $$a0.962$$b2023
000127921 591__ $$aPHARMACOLOGY & PHARMACY$$b85 / 354 = 0.24$$c2023$$dQ1$$eT1
000127921 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1
000127921 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b106 / 313 = 0.339$$c2023$$dQ2$$eT2
000127921 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2023$$dQ1
000127921 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b65 / 189 = 0.344$$c2023$$dQ2$$eT2
000127921 594__ $$a5.2$$b2023
000127921 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000127921 700__ $$aImre, Mirela
000127921 700__ $$aHerman, Viorel
000127921 700__ $$aDebbou-Iouknane, Nedjima
000127921 700__ $$aZemouri-Alioui, Salima
000127921 700__ $$aKhaled, Souad
000127921 700__ $$aBouiche, Cilia
000127921 700__ $$0(orcid)0000-0003-2685-5739$$aNerín, Cristina$$uUniversidad de Zaragoza
000127921 700__ $$aAcaroz, Ula¿
000127921 700__ $$aAyad, Abdelhanine
000127921 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000127921 773__ $$g11, 8 (2023), 2173 [14 pp]$$tBiomedicines$$x2227-9059
000127921 8564_ $$s1499157$$uhttps://zaguan.unizar.es/record/127921/files/texto_completo.pdf$$yVersión publicada
000127921 8564_ $$s2802075$$uhttps://zaguan.unizar.es/record/127921/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000127921 909CO $$ooai:zaguan.unizar.es:127921$$particulos$$pdriver
000127921 951__ $$a2024-11-22-12:03:50
000127921 980__ $$aARTICLE